SPHK2 (NM_020126) Human 3' UTR Clone

CAT#: SC208843

3`UTR clone of sphingosine kinase 2 (SPHK2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SPHK2
• Synonyms: SK-2; SK 2; SPK-2; SPK 2
• ACCN: NM_020126
• Insert Size: 678
• Sequence Data:
  Insert Sequence

  >SC208843 3'UTR clone of NM_020126
  The sequence shown below is from the reference sequence of NM_020126. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTACACTGCTCACTGGGCCTCCTGGCTGCCCGGGGCGGGAGCCCTGAAACTAAACAAGCTTGGTACCCGC
  CGGGGGCGGGGCCTACATTCCAATGGGGCGGAGCCTGAGCTAGGGGGTGTGGCCTGGCTGCTAGAGTTGT
  GGTGGCAGGGGCCCTGGCCCCGTCTCAGGATTGCGCTCGCTTTCATGGGACCAGACGTGATGCTGGAAGG
  TGGGCGTCGTCACGGTTAAAGAGAAATGGGCTCGTCCCGAGGGTAGTGCCTGATCAATGAGGGCGGGGCC
  TGGCGTCTGATCTGGGGCCGCCCTTACGGGGCAGGGCTCAGTCCTGACGCTTGCCACCTGCTCCTACCCG
  GCCAGGATGGCTGAGGGCGGAGTCTATTTTACGCGTCGCCCAATGACAGGACCTGGAATGTACTGGCTGG
  GGTAGGCCTCAGTGAGTCGGCCGGTCAGGGCCCGCAGCCTCGCCCCATCCACTCCGGTGCCTCCATTTAG
  CTGGCCAATCAGCCCAGGAGGGGCAGGTTCCCCGGGGCCGGCGCTAGGATTTGCACTAATGTTCCTCTCC
  CCGCGGGTGGGGGCGGGGAAATTCATATCCCCTGTTCGTCTCATGCGCGTCCTCCGTCCCCAATCTAAAA
  AGCAATTGAAAAGGTCTATGCAATAAAGGCAGTCGCTTCATTCCTCTC
  
  CGGACCGTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_020126.3
• Summary: This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
• Locus ID: 56848