TRAF6 (NM_145803) Human 3' UTR Clone

CAT#: SC209042

3`UTR clone of TNF receptor-associated factor 6 (TRAF6) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TRAF6
• Synonyms: MGC:3310; RNF85
• ACCN: NM_145803
• Insert Size: 700
• Sequence Data:
  Insert Sequence

  >SC209042 3'UTR clone of NM_145803
  The sequence shown below is from the reference sequence of NM_145803. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGTTTTCAGCCACGAAGTACTGATGCAGGGGTATAGCTTGCCCTCACTTGCTCAAAAACAACTACCTGGA
  GAAAACAGTGCCTTTCCTTGCCCTGTTCTCAATAACATGCAAACAAACAAGCCACGGGAAATATGTAATA
  TCTACTAGTGAGTGTTGTTAGAGAGGTCACTTACTATTTCTTCCTGTTACAAATGATCTGAGGCAGTTTT
  TTCCTGGGAATCCACACGTTCCATGCTTTTTCAGAAATGTTAGGCCTGAAGTGCCTGTGGCATGTTGCAG
  CAGCTATTTTGCCAGTTAGTATACCTCTTTGTTGTACTTTCTTGGGCTTTTGCTCTGGTGTATTTTATTG
  TCAGAAAGTCCAGACTCAAGAGTACTAAACTTTTAATAATAATGGATTTTCCTTAAAACTTCAGTCTTTT
  TGTAGTATTATATGTAATATATTAAAAGTGAAAATCACTACCGCCTTGTGCTAGTGCCCTCGAGAAGAGT
  TATTGCTCTAGAAAGTTGAGTTCTCATTTTTTTAACCTGTTATAGATTTCAGAGGATTTGAACCATAATC
  CTTGGAAAACTTAAGTTCTCATTCACCCCAGTTTTTCCTCCAGGTTGTTACTAAGGATATTCAGGGATGA
  GTTTAAACCCTAAATATAACCTTAATTATTTAGTGTAAACATGTCTGTTGAATAATACTTGTTTAAGTGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_145803.1
• Summary: The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from, members of the TNF receptor superfamily. This protein mediates signaling from members of the TNF receptor superfamily as well as the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. This protein also interacts with the transforming growth factor (TGF) beta receptor complex and is required for Smad-independent activation of the JNK and p38 kinases. This protein has an amino terminal RING domain which is followed by four zinc-finger motifs, a central coiled-coil region and a highly conserved carboxyl terminal domain, known as the TRAF-C domain. Two alternatively spliced transcript variants, encoding an identical protein, have been reported. [provided by RefSeq, Feb 2012]
• Locus ID: 7189