UBE2E1 (NM_182666) Human 3' UTR Clone

CAT#: SC209276

3`UTR clone of ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog yeast) (UBE2E1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: UBE2E1
• Synonyms: UBCH6
• ACCN: NM_182666
• Insert Size: 707
• Sequence Data:
  Insert Sequence

  >SC209276 3'UTR clone of NM_182666
  The sequence shown below is from the reference sequence of NM_182666. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGTGGACCAAGAGATACGCTACATAAATTGGGGTTTCACAATTCTTACATTATTTGTCTGTCACAGAAGA
  GAGCTGCTTATGATTTTGAAGGGGTCAGGGAGGGTGGGAGTTGGTAAAGAGTAGGGTATTTCTATAACAG
  ATATTATTCAGTCTTATTTCCTAAGATTTTGTTGTAACTTAAGGTATCTTGCTACAGTAGACAGAATTGG
  TAATAGCAACTTTTAAAATTGTCATTAGTTCTGCAATATTAGCTGAAATGTAGTACAGAAAAGAATGTAC
  ATTTAGACATTTGGGTTCAGTTGCTTGTAGTCTGTAAATTTAAAACAGCTTAATTTGGTACAGGTTACAC
  ATATGGCCATTTATGTAAAGTCCCTCTAAGACTACATACTTTTTGTTTAAAACAAAATTGGAATTTGTTT
  TCCCTTCTTGGAAGGGAACATTGATATTTAACAGAGTTTTTAGAGATTGTCATCTCATATATATAAAATG
  GACACGTGGCTATAAAACACCATATAAGAGATGAGTAGTGCGTTTTATTTTATATGCCAATCTACTTTGT
  TTAAAAAAGGTCTGAATCAGGACTTGTGAAAACCTGTAGTGAAATACCTTAAGCTGTTAACTAACTGTAA
  GGCGTGGAATAGGAGTTGCTCAGTGGATTGGTTCTATGTTGTGGACTACTTAAGTCTGCATTTGTTACTG
  TGCTAAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_182666.1
• Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
• Locus ID: 7324