beta Arrestin 1 (ARRB1) (NM_020251) Human 3' UTR Clone

CAT#: SC209296

3`UTR clone of arrestin beta 1 (ARRB1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ARRB1
• Synonyms: ARB1; ARR1
• ACCN: NM_020251
• Insert Size: 772 bp
• Sequence Data:
  Insert Sequence

  >SC209296 3'UTR clone of NM_020251
  The sequence shown below is from the reference sequence of NM_020251. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGAAGAGGAGGATGGTACCGGCTCTCCACAGCTCAACAACAGATAGACGGGCCGGCCCTGCCTCCACGT
  GGCTCCGGCTCCACTCTCGTGCACTCGGATGCTTACTCGTCTTCTTCCTGTTCTGGTTTCTTTTCCCCTT
  TGTTCTTCCAGTTTCTACCAGGGGGCCCCGTGGGCTTCCAGATCACGGTGATGAACCTCTGGCCTCAGGA
  TTGGCCCCACATCACCACGCCAACAGGACCACAGCGCACTGGCTCCACCCCATCTCTGCCATCTCCACTC
  CCCTCCTTTTCATGCTGTCTCCCAGAAAAGCTGCCAGGGCTCTGGCCTTGGAATTGGACTTGAGATGGGG
  AGCAGACAGGGGAGGATGGGGAATGTGGGACACGGTGTGGTGGGCATGAGGGCTTGGAGGGGTGGGGATG
  AGGGCTCAAGACACGAGAGAAGATGTCCACGGTCCCAGGTGGTTAACAAAGTTCTGGCAGCTAAAAGATG
  ACCGCGTTGAAGGCCACCTCCTTCTGGCTGGGAGGGGCAGAACTGTGGACAGATTCTCAATGCCTTTTTG
  AAGTTCTGACCCACCAAAGACCTTCTGCCTTCACCCTCCTCCCCACCTGATGTCCCTCTGTGTCTGATAG
  TGATGTTGGTGAAAGTTCGTAGACCCCAGGAGTAGAGAAAAGCAACTGGACTGACTTTCTTACCAGCAGT
  TACCTAGACTGAGGCAAGCTGTGTGGACTCACCCAAGTATATTTCAGTACTGTCAGGCTGTGACATCTTA
  GC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_020251.2
• Summary: 'Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011]'
• Locus ID: 408