ASK1 (MAP3K5) (NM_005923) Human 3' UTR Clone

CAT#: SC209333

3`UTR clone of mitogen-activated protein kinase kinase kinase 5 (MAP3K5) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MAP3K5
• Synonyms: ASK1; MAPKKK5; MEKK5
• ACCN: NM_005923
• Insert Size: 732 bp
• Sequence Data:
  Insert Sequence

  >SC209333 3'UTR clone of NM_005923
  The sequence shown below is from the reference sequence of NM_005923. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCACACTGTGGAAGGCTATCATTGACTTTCGAAACAAACAGACTTGACTGTTGCTCAATCTAATCTTCGA
  TGGAAATTCTAAAAATTAATACAGAGCTGATCTTCTTGGGGGTGGGAAAATCGAAGGGAGAGGAGAAAGG
  CGCTGCACTTTAAATCCAGTATTTGTTTACTCATGTTAAAAAAAAAAAAAACAGACAAAACACACTGAAA
  TTTCCTAACTACATCTATTTCTATAATTTTTAAGGACTCTTCATAAGGACTCTTAAAATAATCCTGAACA
  TTAGAACCCTAATGTTCAGGAAGATTTTAATCTAAGCATTTTTATGGAAATATTTTTAATGCAGCAGCTA
  TTGCACTTCAGCCAAATGTTTATTTCACACAAAACGGATGTAACATTTCATGTGATCGTGCACCACTGGA
  ACAAAACCAAAATGTGACCATAACTGTTTAGGCTTCTGTGTGTTTGTAATATGCTCTAATAATCTGAGTA
  GAAATGCGTAATTTCAATTACTGTATAAAGTTTATGTTTTTTTAAGTGTGCAGAATCTGAGAGCAATGGT
  TTTTACTTCTCTGTGTTAATTGTAATATTGACTCTATTTTGTAACTTAAGTTTCTGACCTGTCGTACATT
  TGTTTGAGTCGTTTATGTACTACTGAACTGTACCAGTTGCACATGCTTGAACTGTAGTAATGTTAGCTTG
  TTCTAAAGCTATCCATTGTGTCATATTTACTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005923.3
• Summary: 'Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]'
• Locus ID: 4217