QDPR (NM_000320) Human 3' UTR Clone

CAT#: SC209494

3`UTR clone of quinoid dihydropteridine reductase (QDPR) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: QDPR
• Synonyms: DHPR; HDHPR; PKU2; SDR33C1
• ACCN: NM_000320
• Insert Size: 754 bp
• Sequence Data:
  Insert Sequence

  >SC209494 3'UTR clone of NM_000320
  The sequence shown below is from the reference sequence of NM_000320. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGTAACCACAGAAGGAAGGACGGAACTCACCCCAGCATATTTTTAGGCCTCATCTCAGTGCCTATGAGGG
  GCCTGCCAGAAAAGTCACTAACCTGTCTCAGTGTGGCCTTGTCCAGCCTTGTGTTTTCTGTAACCCCTGT
  TTGTGGTACGAGATAATGAGTCCTATTTTTCTCTCACATAATATGCATTTGCTCTCCTAGGACAGTGTAA
  TACATTTATGTGAAGTAAAGACATGCGAGACTGGTGGCCTGCAAATAGCATCCGTTGATCTGTGTTAACT
  GCATAGGGAGGGCTCTGCATAGCACCTGCTATAGCGGTGTCATGTTGGATCGCTTTTGTGACTGTTCATC
  TGTCCTTGACAGTGGCTGTCATCTTGACTACTTTGTTGATTTGTTGGTATTGGGGACATTTTAAAGGCTG
  AGTTATTTTTGAATGTCATGTTTATGTCATAGACGTAGTTTTCGCATCCTTGAATTAAACTGCCTTAACT
  CCTTTTGTGGTATAAGCAAAACTACATGGACTCTGTCCTGGTATCCTTTTCCTGTGTGGTTGCCCCGTGT
  CCTCTGGCCTAGGGTTAAGTGTGCAAGATAACTACTCGTGAGTATTCAGAATGTTGTTCCTAATAAATGC
  ACTTGTTGTCTGTCTTCTTTAATCAAATCACATCTTATATACAGCAGTCAGAGATGAGTATACTAGAATC
  ATGGATTGCTGGAGGTCTTTTAATCTGATGTTCTCAGAAGGGGGTGGATTTAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000320.2
• Summary: 'This gene encodes the enzyme dihydropteridine reductase, which catalyzes the NADH-mediated reduction of quinonoid dihydrobiopterin. This enzyme is an essential component of the pterin-dependent aromatic amino acid hydroxylating systems. Mutations in this gene resulting in QDPR deficiency include aberrant splicing, amino acid substitutions, insertions, or premature terminations. Dihydropteridine reductase deficiency presents as atypical phenylketonuria due to insufficient production of biopterin, a cofactor for phenylalanine hydroxylase. [provided by RefSeq, Jul 2008]'
• Locus ID: 5860