S adenosylhomocysteine hydrolase (AHCY) (NM_001161766) Human 3' UTR Clone

CAT#: SC209750

3`UTR clone of adenosylhomocysteinase (AHCY) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: AHCY
• Synonyms: adoHcyase; SAHH
• ACCN: NM_001161766
• Insert Size: 762 bp
• Sequence Data:
  Insert Sequence

  >SC209750 3'UTR clone of NM_001161766
  The sequence shown below is from the reference sequence of NM_001161766. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGATCACTACCGCTACTGAGAGCCAGGTCTGCGTTTCACCCTCCAGCTGCTGTCCTTGCCCAGGCCCCAC
  CTCTCCTCCCTAAGAGCTAATGGCACCAACTTTGTGATTGGTTTGTCAGTGTCCCCCATCGACTCTCTGG
  GGCTGATCACTTAGTTTTTGGCCTCTGCTGCAGCCGTCATACTGTTCCAAATGTGGCAGCGGGAACAGAG
  TACCCTCTTCAAGCCCCGGTCATGATGGAGGTCCCAGCCACAGGGAACCATGAGCTCAGTGGTCTTGGAA
  CAGCTCACTAAGTCAGTCCTTCCTTAGCCTGGAAGTCAGTAGTGGAGTCACAAAGCCCATGTGTTTTGCC
  ATCTAGGCCTTCACCTGGTCTGTGGACTTATACCTGTGTGCTTGGTTTACAGGTCCAGTGGTTCTTCAGC
  CCATGACAGATGAGAAGGGGCTATATTGAAGGGCAAAGAGGAACTGTTGTTTGAATTTTCCTGAGAGCCT
  GGCTTAGTGCTGGGCCTTCTCTTAAACCTCATTACAATGAGGTTAGTACTTTTAGTCCCTGTTTTACAGG
  GGTTAGAATAGACTGTTAAGGGGCAACTGAGAAAGAACAGAGAAGTGACAGCTAGGGGTTGAGAGGGGCC
  AGAAAAACATGAATGCAGGCAGATTTCGTGAAATCTGCCACCACTTTATAACCAGATGGTTCCTTTCACA
  ACCCTGGGTCAAAAAGAGAATAATTTGGCCTATAATGTTAAAAGAAAGCAGGAAGGTGGGTA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001161766.1
• Summary: 'S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]'
• Locus ID: 191