PIGH (NM_004569) Human 3' UTR Clone

CAT#: SC209758

3`UTR clone of phosphatidylinositol glycan anchor biosynthesis class H (PIGH) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PIGH
• Synonyms: GPI-H
• ACCN: NM_004569
• Insert Size: 778 bp
• Sequence Data:
  Insert Sequence

  >SC209758 3'UTR clone of NM_004569
  The sequence shown below is from the reference sequence of NM_004569. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CACCAGAAAGCCACATCAACAAGCCCATGAGCCCCAGCGTTCAGAAGGCCAGCATTGTCTTCCATGGGAG
  ATGACTCTTAAGCCATAGGGGCTGGTTTTCCGTACTCCAAACCATCAGGTGGACACAGTCCTAGGAACCA
  TTATGGATGTAGTGCATCTTAGAGCCATAGAGCAGGTGACTGGAAATCTAACTCAGTATATTTCCGTGTA
  TTATAGTGTTTTCCTTGTAAGGTTTTGCCTACTTTACCAAAGGAGGGGAGACCTTAAGAATTTTGACACA
  GTATGTCAAAAGTAATGTCAGCAAAGAACACTTTCGGAAATTGCTAAGCATGTTCAGGTTTACTTTGTTG
  ATGTTTGTGAAGCAAAACAATGGGAAACTGACATCAGATGTCTTGAGAAAGCTATATTTTCCAATAGTAC
  CCCTTGTGTAAAGTTACGAAAAAAACAAGCCCTTCAGTACTGGTTAGCAGGAAGAAATGTCCTACAAAAG
  ACGAGTCTGTCAACCTAGTGCCTGTGTTCTGTACAGGGCTTATTTATTTACTGTTAATAAACAGATCTTC
  CAAAACACACAGTTACCTGGTTCACAGATAGCTGCTTTTTATTGACAAACAAGATCATAGTATTTCAGTC
  ATGGTGTCAAGCACATTAATACTTTTCTGTTACTCAGCATTTTTTTTTATATCAGTTCAAAAGCTACTTT
  TGCCAAGAAGGATGAGACAAAAGGCCAGACAGAAATTGGACTTTGGTTTTCAGACATGGGACTCTCATGT
  TAACAAAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004569.3
• Summary: 'This gene encodes an endoplasmic reticulum associated protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. The protein encoded by this gene is a subunit of the GPI N-acetylglucosaminyl (GlcNAc) transferase that transfers GlcNAc to phosphatidylinositol (PI) on the cytoplasmic side of the endoplasmic reticulum. [provided by RefSeq, Jul 2008]'
• Locus ID: 5283