ERCC8 (NM_000082) Human 3' UTR Clone

CAT#: SC209828

3`UTR clone of excision repair cross-complementing rodent repair deficiency complementation group 8 (ERCC8) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ERCC8
• Synonyms: CKN1; CSA; UVSS2
• ACCN: NM_000082
• Insert Size: 800 bp
• Sequence Data:
  Insert Sequence

  >SC209828 3'UTR clone of NM_000082
  The sequence shown below is from the reference sequence of NM_000082. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCCTTTGAAGATGCCTGGAGCAGCAGTGATGAAGAAGGATGAATATCATCTTTAGTACCTTTTTGTCTCT
  GCTGAAACTTTTTAAATGAGACTGTGTTTTTTTCAACTGTATGGTCTATTCCTGACAGCTAAATTAGCCC
  TAAATGTGGGTAATATTTTTCCTCATGTTTTAAAATGAGGTTAATATTTGCATAAAATCCTAAAACAGAC
  TTCTGTATAGTTTATTTAGTCAAAATGTGTTCCTTGATCCCAGATGTTGTGGCCTGGGAAAGCCCTCATT
  GCTACAGTACAAGTAACACAAGTCGTTGTACCTCAGTTGTGACCTTCAGCAGATTTTATGAACTATAAGA
  TGCAGTCTCAGAGGATCAGCAAGTGGAGGCCATCAGTATTGACTTTCTCTTACTTGCTGTACTATCAGCC
  TGCTCGTTTCCACCTTTAAGAATGATTTTGCCAAGAATGATTATATCAAAAATAGTAGTTGAAATGGTAA
  CATCAAAATTATTTTATTCTTTCTTCTTCATGTATTCACATTTTTCAGTGGTTTCATTTAATTAACCATG
  CTTTATGTTAAACATTTTGGGGCTCAATGTCTCCTACTATCCAAAATGTGCATCACAGGAGGCTTTTAAC
  TTTGTGAAAATCCCATGTTTGCTTTATTTTATTTTAATGTCAGAAGGCAGTTTGCGCTAATGCTTGAACT
  CTTTTTCTGTGAAACTCATTAAGGTATGACCAAATCCTGCCTCATTAATTCAAGCAGAAAATATCCTGGC
  AGGGAATCTGGCTTAAACATGAAATGCTGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000082.3
• Summary: 'This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]'
• Locus ID: 1161