FLT3 (NM_004119) Human 3' UTR Clone

CAT#: SC209844

3`UTR clone of fms-related tyrosine kinase 3 (FLT3) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: FLT3
• Synonyms: CD135; FLK-2; FLK2; STK1
• ACCN: NM_004119
• Insert Size: 772 bp
• Sequence Data:
  Insert Sequence

  >SC209844 3'UTR clone of NM_004119
  The sequence shown below is from the reference sequence of NM_004119. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGCTCAGGTCGAAGATTCGTAGAGGAACAATTTAGTTTTAAGGACTTCATCCCTCCACCTATCCCTAAC
  AGGCTGTAGATTACCAAAACAAGATTAATTTCATCACTAAAAGAAAATCTATTATCAACTGCTGCTTCAC
  CAGACTTTTCTCTAGAAGCTGTCTGCGTTTACTCTTGTTTTCAAAGGGACTTTTGTAAAATCAAATCATC
  CTGTCACAAGGCAGGAGGAGCTGATAATGAACTTTATTGGAGCATTGATCTGCATCCAAGGCCTTCTCAG
  GCTGGCTTGAGTGAATTGTGTACCTGAAGTACAGTATATTCTTGTAAATACATAAAACAAAAGCATTTTG
  CTAAGGAGAAGCTAATATGATTTTTTAAGTCTATGTTTTAAAATAATATGTAAATTTTTCAGCTATTTAG
  TGATATATTTTATGGGTGGGAATAAAATTTCTACTACAGAATTGCCCATTATTGAATTATTTACATGGTA
  TAATTAGGGCAAGTCTTAACTGGAGTTCACGAACCCCCTGAAATTGTGCACCCATAGCCACCTACACATT
  CCTTCCAGAGCACGTGTGCTTTTACCCCAAGATACAAGGAATGTGTAGGCAGCTATGGTTGTCACAGCCT
  AAGATTTCTGCAACAACAGGGGTTGTATTGGGGGAAGTTTATAATGAATAGGTGTTCTACCATAAAGAGT
  AATACATCACCTAGACACTTTGGCGGCCTTCCCAGACTCAGGGCCAGTCAGAAGTAACATGGAGGATTAG
  TA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004119.2
• Summary: 'This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq, Jan 2015]'
• Locus ID: 2322