ACAT1 (NM_000019) Human 3' UTR Clone

CAT#: SC209895

3`UTR clone of acetyl-Coenzyme A acetyltransferase 1 (ACAT1) nuclear gene encoding mitochondrial protein for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ACAT1
• Synonyms: ACAT; MAT; T2; THIL
• ACCN: NM_000019
• Insert Size: 792 bp
• Sequence Data:
  Insert Sequence

  >SC209895 3'UTR clone of NM_000019
  The sequence shown below is from the reference sequence of NM_000019. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGAGGTGCTTCTGCCATGCTAATTCAGAAGCTGTAGACAACCTCTGCTATTTAAGGAGACAACCCTATG
  TGACCAGAAGGCCTGCTGTAATCAGTGTGACTACTGTGGGTCAGCTTATATTCAGATAAGCTGTTTCATT
  TTTTATTATTTTCTATGTTAACTTTTAAAAATCAAAATGATGAAATCCCAAAACATTTTGAAATTAAAAA
  TAAATTTCTTCTTCTGCTTTTTTCTTGGTAACCTTGAAAAGTTTGATACATTTTTGCATTCTGAGTCTAT
  ACTTATCGAAATATGGTAGAAATACCAATGTGTAATATTAGTGACTTACATAAGTAGCTAGAAGTTTCCA
  TTTGTGAGAACACATTTATATTTTTGAGGATTGTTAAAGGTCAAGTGAATGCTCTTTATAGGTAATTTAC
  ATTTAGTAAATTACGGTAAATTAAATTACTTCTCTTTACAGTAAGAGTTGGCTATTCTGGACAAACTAGC
  AGTGCTTCATATAATCACTCAAACCACAGTGTGTGCAGCAGTACTAGAAACAAGACAGAAGCCCATGTCC
  TCAGGGTCTAGAGTGGGGGCAATTTCTTATAACCTCAACATTCAGGGTTGGGGGAGGTCAAGCAGAAAAC
  CCTGGAGTTTGGGCTCTGAATTACTATAGCAGCATAGAGAGTGGGAAGGGAGGTAGAAACTGATATGCTG
  AATGGATATATAAAAAAGGGAACAGATCACCACTTCCAATACACGACAATGCCTGTTCTTAAGCAGGACA
  GACTGTAACAGAAGTATCTCGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000019.3
• Summary: This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]
• Locus ID: 38