LMO2 (NM_001142315) Human 3' UTR Clone

CAT#: SC209901

3`UTR clone of LIM domain only 2 (rhombotin-like 1) (LMO2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: LMO2
• Synonyms: LMO-2; RBTN2; RBTNL1; RHOM2; TTG2
• ACCN: NM_001142315
• Insert Size: 805 bp
• Sequence Data:
  Insert Sequence

  >SC209901 3'UTR clone of NM_001142315
  The sequence shown below is from the reference sequence of NM_001142315. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACAGGACATCTACGAGTGGACTAAGATCAATGGGATGATATAGGCCCGAGTCCCCGGGCATCTTTGGGGA
  GGTGTTCACTGAAGACGCCGTCTCCATGGCATCTTCGTCTTCACTCTTAGGCACTTTGGGGGTTTGAGGG
  TGGGGTAAGGGATTTCTTAGGGGATGGTAGACCTTTATTGGGTATCAAGACATAGCATCCAAGTGGCATA
  ATTCAGGGGCTGACACTTCAAGGTGACAGAAGGACCAGCCCTTGAGGGAGAACTTATGGCCACAGCCCAT
  CCATAGTAACTGACATGATTAGCAGAAGAAAGGAACATTTAGGGGCAAGCAGGCGCTGTGCTATCATGAT
  GGAATTTCATATCTACAGATAGAGAGTTGTTGTGTACAGACTTGTTGTGACTTTGACGCTTGCGAACTAG
  AGATGTGCAATTGATTTCTTTTCTTCCTGGCTTTTTAACTCCCCTGTTTCAATCACTGTCCTCCACACAA
  GGGAAGGACAGAAAGGAGAGTGGCCATTCTTTTTTTCTTGGCCCCCTTCCCAAGGCCTTAAGCTTTGGAC
  CCAAGGAAAACTGCATGGAGACGCATTTCGGTTGAGAATGGAAACCACAACTTTTAACCAAACAATTATT
  TAAAGCAATGCTGATGAATCACTGTTTTTAGACACCTTCATTTTGAGGGGAGGAGTTCCACAGATTGTTT
  CTATACAAATATAAATCTTAAAAAGTTGTTCAACTATTTTATTATCCTAGATTATATCAAAGTATTTGTC
  GTGTGTAGAAAAAAAAACAGCTCTGCAGGCTTAAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001142315.1
• Summary: 'LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number T-cell acute lymphoblastic leukemia-specific translocations occur. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Nov 2008]'
• Locus ID: 4005