BPGM (NM_199186) Human 3' UTR Clone

CAT#: SC210004

3`UTR clone of 23-bisphosphoglycerate mutase (BPGM) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: BPGM
• Synonyms: DPGM; ECYT8
• ACCN: NM_199186
• Insert Size: 819 bp
• Sequence Data:
  Insert Sequence

  >SC210004 3'UTR clone of NM_199186
  The sequence shown below is from the reference sequence of NM_199186. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTAGAAGATCAAGGAAAAGTGAAACAAGCTAAAAAATAGTCTTTCTCAACTGTTGGCTAAGAAGAAATGC
  AAAAGAAGTGGCATAGGAGTGTGTTATGGGTGCTGAACTCTCTCTCTTTTTCCCCGATTTTCCAGAGCTA
  GGCTGTGGAGTAGAGTTTGTATAGGTAACTAGGTAACTTATTGTGGCCCAGATAAGGCTTTAGGATGCCT
  CAGTGCTTATGTCATAGCCTTATGAGTTAGCTTTCTTGCTAGCCCCCTAGTCGGTCACCAAACTAGTAAC
  TAGTGGGGCTTAATGAAGGTCATAAGTTTCTGAGATGGGAGAGCAACAAGTAGAGATGAAGTTAAAGGTA
  TTTATCATTCAAGAAATCATTATTGAGTCACCATTGACAGGCACTATTCTAATCAGTAGTTCACTTTAAT
  ATTTAATAAGATTTTCTGGGATAACAGTAAGGGATATTAGATAATATACCGTATGTATTTATTACTAGTC
  TTTTCCTCTAGGAAAAGGGATACTTTGATAATTAAGGCCAGAGGCCCATTAGTTGAGAAAGTCACAGATA
  TATTTCTCCAAGAAAGCCAACAACCACCACCACAATGACAGAAATGACAACAAGGCCCTTTAACTTGTCT
  TCTAGTTTAGAGACATCCTTCATTTGACATTTAGTAGAATTCCTCTTTGGCCACAAGAATAAGCAGCAAA
  TAAACAACTATGGCTGTTGAGGTTCTCATTTTGGTTTGTTTTAATTTTTTGAACTTTGGGTACCTGTAAT
  TAGTTTAAAAATAAAGTTCCTGATAATAAAGTGACTGAAAATGGCATCC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_199186.2
• Summary: '2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009]'
• Locus ID: 669