ACK1 (TNK2) (NM_005781) Human 3' UTR Clone

CAT#: SC210124

3`UTR clone of tyrosine kinase non-receptor 2 (TNK2) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TNK2
• Synonyms: ACK; ACK-1; ACK1; p21cdc42Hs
• ACCN: NM_005781
• Insert Size: 835
• Sequence Data:
  Insert Sequence

  >SC210124 3'UTR clone of NM_005781
  The sequence shown below is from the reference sequence of NM_005781. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGCCACCTTCTGGGCTCCTGGGGCCCTGCCCACCACAAGCGCTGAGATGCGTCTGGAGAGCCAGAGGGC
  CTGCCTGAAGGAATCACCTGAGCCTGTCCGTCCACCAGGAGTGGGGAGATGCCCCCATCCAGTCCTGGAG
  GACCCGCTGCTCCTGCTGCTCCCGGGGATGGAGCAAGGCCAAGGCTGCGGGAGGCTGGGAGCCCTGCCCT
  GCCCATCCCTCCTGCACCAGCGCTGTCCCTGCACACTTTGGTTCAGTCCCGGTGCCCCTGCCAAGATGTG
  GAAGGGGCCGGGTGAAGACAGGCTTGAGGGCTGCCCCAGCAGGCTCTGGGTATGACCTGCCTCTGGCCCT
  GGTCCTGGGCGGGGCCTGTGGGTGGAGTAGTACCCCCAGGCCCTGCCCTGGGTGACAGACTGGGAGGAAA
  CCAGGCTGGACCTGGGCAGGCGGGATGTGTTGGCCACAGGGAGAGGCGGACCGGCACCCGGTGGGACCTC
  CTAGGACTGGGCCTTCTTCCAGGGGGCCCCTGGCAGCAGCTGGGGTGTCGGGCAGAATGTGACTTGTGGC
  CTTACCATGGACTTGAATGGGACTTGGCTGGCCTCAGGATCTTGTGCCTGGAAATAGCCTGAGGTGGCTC
  AGGAAGCGGAGAAAGGGTGCCAGACCATTCTCTGGCGGGGACCAGGGCCCAAGGCCCCAGGGCTGGAAGG
  AGACCAAGGGGCAGCCGCCCTGGAGGGACATCAGTGCTTCCTCTTCCACCCAATTCCCCCACGCGGTTCC
  ATGTTTTCCCACCAGCCTGTTGGCGAAGTTGCTGCTCCGGCATTCAGTACCTGCTTCTTCCAGAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_005781.4
• Summary: This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]
• Locus ID: 10188