Aspartate beta hydroxylase (ASPH) (NM_001164756) Human 3' UTR Clone

CAT#: SC210271

3`UTR clone of aspartate beta-hydroxylase (ASPH) transcript variant 12 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ASPH
• Synonyms: AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN; junctin
• ACCN: NM_001164756
• Insert Size: 837 bp
• Sequence Data:
  Insert Sequence

  >SC210271 3'UTR clone of NM_001164756
  The sequence shown below is from the reference sequence of NM_001164756. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGTTCAGGGAAAGACTTGTGTCATATTGGATTTACATAACCAGTAACCTTGATTCAGGGACTGAAGTCAT
  TGGCTAATGAACACCTGAAGCAGCCTCCTTTTTCTTTTCTTTCCTTGGCTTATGCAGGGCTTAATGTGCA
  GTGGGGTGGTTGTGATCTTACCGTGCAAGTCAACCATGTGATCTTGCCCAGTACAGCTACTAGCTAGTCC
  CTTGCTCGCTCAGCTCCCCCAACTTCTATTGAAGAAAATGGTACTCCTCATTCTTGTAGTCAGCTACAAA
  GTACACTGAAAATGATGTTCTTGGTGGTATAATTGGTTTCTGTATCGTTTTGTTTCAACTCATGTATTCA
  CTGAACTAAATTTGGACACTTAACAGCAAATTGTGTTGTGGTTAACCCTTGATGCTTGTCTTTCTAACAC
  ACTATTAATTATGATGATTCTAATGGATTTCATTATAAAAATATTTCTGGCATGATTTTTAAGTTAAATG
  CTTCTCTGTTCTTTAACATGACTGATGTATAAAATGATGGTTCTTTACTAAGCTGATATTTTTTATTGTA
  ATTTGTTTAGGTTTGTCAGATAGGTTCATACAAATTTCTATGTAAAATTCTGTGTTAATGGTGCTTTTAA
  AATAATTTAAAAATAACTCCATGTTTTTGCCTTAGAGTAAGTTAACTTACTGTTTTCAGATAGTAGCATG
  ACATATTTCTGTCTGTGAAAGCAAAATTTATTTTAAATTTTATTTCCAAATATACATCCAGAGAAAGTAA
  TTTGTATTTTTTTTAAAGTAGGCATATTACACAAGAGGGAACATGTGAATATGTATCTTAATGTTGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001164756.1
• Summary: 'This gene is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis. [provided by RefSeq, Sep 2009]'
• Locus ID: 444