PIGZ (NM_025163) Human 3' UTR Clone

CAT#: SC210295

3`UTR clone of phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PIGZ
• Synonyms: GPI-MT-IV; PIG-Z; SMP3
• ACCN: NM_025163
• Insert Size: 851
• Sequence Data:
  Insert Sequence

  >SC210295 3'UTR clone of NM_025163
  The sequence shown below is from the reference sequence of NM_025163. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCTCAGTCTTCACATTGTGGAGCTGGGGGAAGAAACCTGACAATATGACAGAGCACCCACTGCCCAAGAC
  TCAGCCATAGAAGATGCCGCCCCACCTTCTACTTGGGTAGCTGGGCTGGGACGCTGGGACAGGACCCCGT
  CCTCCTTCATGACTCCCACTGCTGCCTCTCCTGGGCATGGCTGTTAGCTGTTCTGCCTTCTGGGTGAGCT
  GGCACTCTTCTCCCTGAGACCAAAGATTTGACCTGTCGGTCTTGATGTCAAGGTCCCCAAAGACCAGGTT
  AAGTGACGACACCTGTCTGTTCCTGCCCTGTTGCTTCCAGCCACTGTGATGTTTGAAATATGTGATAGTA
  CCTGGTTGTGAAAAAAGACAATGAACTGCTAGTGACATTCCTCAATGACCTCTCCCAAACCTCCCATGAT
  GCCTTACCCTTGCTGTCATGACAACCCTCTGGCTTCCTAAGACCCATCTGCCTATCGAAATATGTGCAAG
  TCAGTGAGACGAAGTATAGAGAACAGGTGGCCCAGATCCAGGGGACCCAACTTCTGGCCCCTTGGTCTGT
  CACCTCCTCGCTGTGTGATCTTGAGAAAGCTCCTTCCACTCACCCACCCCACTTCCCAGTCTGTTGGGAT
  CAGAGGAACTTTGAGGTGTCTGCCGGCTAACATTGTGTCATTCCTGGAGTCCACAGTACACGTCCCCTGC
  CTCAACAGGCACAGCTCTCACAAAGCTCTTCAAGCATGGAAGTGGGAGTTGTGTTGTACTTCATGGCACT
  CTGATGCCTGCTGTCTCAGTGTTTGGTTATTATGCAAACAAGTAATGTTTGAAATATATAATAGCACTGG
  AGATTTTCTCC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_025163.2
• Summary: The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. This gene encodes a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors. [provided by RefSeq, Jul 2008]
• Locus ID: 80235