DPH2 (NM_001039589) Human 3' UTR Clone

CAT#: SC210429

3`UTR clone of DPH2 homolog (S. cerevisiae) (DPH2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DPH2
• Synonyms: DPH2L2
• ACCN: NM_001039589
• Insert Size: 848 bp
• Sequence Data:
  Insert Sequence

  >SC210429 3'UTR clone of NM_001039589
  The sequence shown below is from the reference sequence of NM_001039589. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGATTGCCATCGCCTATGAGGATGAGGGAAGCGGCTGATACCATGTGGGGCTGGAGACATAGATGGACTT
  ATGAATGGCTGCTAGGACCTTTAGTGCTCCCTGCACCAACCTCCCATCCCCCTGCCAAGATCCTTGAAGG
  ACCCTGGAAGGAGGGAGAGCAGGCAGCCCTTCACAGGATAGGATCCGTCTCTGTCCTGTCCTGGCACTGG
  CACAAGCTCAGCACATGCCCAGTAATGCGTGTTGTTTGGCTGATGGAATAAAGGGCTTAGGGACTTCCCT
  GAGGCCTCTGGACCCATCTGTCTTCCTGAGGGCAGCCCAGGACCTTTGGCCAATCCCAGTTCCCAGGCTG
  CAGTTGAGGGTCTGTCCTTGTCAAAAGGCAGGTGCTAGACAGTCTAGACCAGGGTTTCTCAAACTCGTAC
  TTGACATTTGGGGCCAGATAATTCTTTGTTGTGGGGCTGTCTGGTGTATGGTAGGGTGCTCAGCAGCATC
  CCTGGCCTCTGCCCACTAGACATCAGAAGCACTCCCCCAGTTGTGACAACCAAAAATATCTCCAGACCTT
  GGCAAATGTTATCTGTGGGGGAAAATTGCCCTCAATTGAGAACCACTGGTCTAGCTAGACCTGCACTGTC
  CAGTACAGTAGCCACTAAATACATGTGGCTAAACTTAAATTTAAGTTAATTAAGATTAAAAGCTCAGTTT
  CTCAGTCACATTAGTCATTCAAGTGTTCAGACAGCCACATGAGGGGACAGTGCAGCTACAGGATATGCCA
  TCATGGCAGAAAGTTCTATTGGTTGGACAGCGTTGGTCTATACTGACTCTTATTTCTCAGGGAGATCACA
  GCAACCTA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001039589.1
• Summary: 'This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]'
• Locus ID: 1802