PAH (NM_000277) Human 3' UTR Clone

CAT#: SC210433

3`UTR clone of phenylalanine hydroxylase (PAH) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PAH
• Synonyms: PH; PKU; PKU1
• ACCN: NM_000277
• Insert Size: 871 bp
• Sequence Data:
  Insert Sequence

  >SC210433 3'UTR clone of NM_000277
  The sequence shown below is from the reference sequence of NM_000277. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TTTGCAGTGCCCTCCAGAAAATAAAGTAAAGCCATGGACAGAATGTGGTCTGTCAGCTGTGAATCTGTTG
  ATGGAGATCCAACTATTTCTTTCATCAGAAAAAGTCCGAAAAGCAAACCTTAATTTGAAATAACAGCCTT
  AAATCCTTTACAAGATGGAGAAACAACAAATAAGTCAAAATAATCTGAAATGACAGGATATGAGTACATA
  CTCAAGAGCATAATGGTAAATCTTTTGGGGTCATCTTTGATTTAGAGATGATAATCCCATACTCTCAATT
  GAGTTAAATCAGTAATCTGTCGCATTTCATCAAGATTAATTAAAATTTGGGACCTGCTTCATTCAAGCTT
  CATATATGCTTTGCAGAGAACTCATAAAGGAGCATATAAGGCTAAATGTAAAACACAAGACTGTCATTAG
  AATTGAATTATTGGGCTTAATATAAATCGTAACCTATGAAGTTTATTTTCTATTTTAGTTAACTATGATT
  CCAATTACTACTTTGTTATTGTACCTAAGTAAATTTTCTTTAGGTCAGAAGCCCATTAAAATAGTTACAA
  GCATTGAACTTCTTTAGTATTATATTAATATAAAAACATTTTTGTATGTTTTATTGTAATCATAAATACT
  GCTGTATAAGGTAATAAAACTCTGCACCTAATCCCCATAACTTCCAGTATCATTTTCCAATTAATTATCA
  AGTCTGTTTTGGGAAACACTTTGAGGACATTTATGATGCAGCAGATGTTGACTAAAGGCTTGGTTGGTAG
  ATATTCAGGAAATGTTCACTGAATAAATAAGTAAATACATTATTGAAAAGCAAATCTGTATAAATGTGAA
  ATTTTTATTTGTATTAGTAATAAAACATTAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000277.1
• Summary: 'This gene encodes a member of the biopterin-dependent aromatic amino acid hydroxylase protein family. The encoded phenylalanine hydroxylase enzyme hydroxylates phenylalanine to tyrosine and is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. [provided by RefSeq, Aug 2017]'
• Locus ID: 5053