DGKB (NM_145695) Human 3' UTR Clone

CAT#: SC210458

3`UTR clone of diacylglycerol kinase beta 90kDa (DGKB) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DGKB
• Synonyms: DAGK2; DGK; DGK-BETA
• ACCN: NM_145695
• Insert Size: 837 bp
• Sequence Data:
  Insert Sequence

  >SC210458 3'UTR clone of NM_145695
  The sequence shown below is from the reference sequence of NM_145695. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CATGCACAGTGAGTACAGAGTAGTTGATATGCTATGTCAATCTCAGTTTTGCTTTCCTCTTTGACTAAAT
  AACCACAATAACTGATTTTTTTCTTTATTTCTTTTCAACCTATCAGCAAATAGTCTTTTTGTTGTTGTTG
  TTATGTGTGTGTCAGAGCCACTACATTTAGGTTGTAGACATTATATACCCTTGGCAATGATTTAGCTCTT
  GAATGTTTGTGTTAGCCTAAGTATAAATAGATCTTTTAAATAGATCAATTATAAACCATAGATCAATTAT
  AAACTATGGAGCTAAACAAAATATTAATAAAAGTTTATCTGAAACTTTTTTGTTTATTTCAGAGCACATT
  ATTAGAATATTATTTGCAAGAAATGCAGACCTAAGCTTATATGTGAACTTATTTCTCAGCTTTTCTATGC
  CTCCATTTGGGGATTTGAGGGCTTTCTTCTCCATAAGAAAAAAATTTCTCTCCAGTTTCTACCATAATTA
  ATTGTGTTTTCCAGAATGAGGTATTATTTAAGGCAGACACTGCCCCTCTCAAAAAAAAATCAGTTTTCAT
  TTGCATAGTGAATATTTTATTGCATTTCAAAAACATGCTAGGAACTGCTTTTGGCACTGGGAGTAGACAC
  ATGAACAAGACCAACAGTGTAATTTCCTTCAAGTTACTTACATTCCTATAATAGAGGACCGAATAAATAA
  ACAACTACATGATAAATATAACTTCAGACTGTGAGAGTTATTAAAAAATAAGGTGAAATGATGATAAGAA
  GCTGGATTAGGTGTGGAGAATAAATACTACTTGAGATAAGGGAGACCTCTTTGAAAGGACATAGCCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_145695.2
• Summary: 'Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017]'
• Locus ID: 1607