TAF5 (NM_006951) Human 3' UTR Clone

CAT#: SC210518

3`UTR clone of TAF5 RNA polymerase II TATA box binding protein (TBP)-associated factor 100kDa (TAF5) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TAF5
• Synonyms: TAF(II)100; TAF2D; TAFII-100; TAFII100
• ACCN: NM_006951
• Insert Size: 876 bp
• Sequence Data:
  Insert Sequence

  >SC210518 3'UTR clone of NM_006951
  The sequence shown below is from the reference sequence of NM_006951. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAAACCTGGTTCTAGCTGCAGGAGCTTATAGTCCACAATAAACCATCGGTATTAAAGACCTTTTGGAAGC
  TACTGTTTTTAAAAAGGGAGACTAAAAGCAAATACCTCAGTGATTAATATTTAAGCTACAGAGAATGTTT
  TTGTCTATATGGATCTGGAAGTATGCTGCTTGGAAAAATCTGAACAGGACAGTTCCACGTTTCTATAGCA
  ACCACATTTGACTAATTTCCGTTAGTTGAATAAGAGGTATTATGATCATGGAGGGGACATTTATGGTGCT
  TTGGATTGTGTGGAAACTATGCATTTTCTGTTCAAATGCTATTTTAATTTATTACATTTAGAAAAAAAGT
  TGATTTCAATAATTCATCCTGCTTCAAGATTCAAATTCAGAAATATACTATCATCTTGAATTTTAGCTGA
  AGAATCCTATGAGCATGTATGTTTCTGCTGTAAAAACGTAGTTACTGTATGGCACTCAAAAACTATGTTA
  AATGATCCACTAACTTTTTTTTTCTTGGCCCATGATTAATGGAATGTATGTAACTAGGTAGGGTTCCTTT
  CTTAGATCTAGAGGAAGTACAGCCACCCACTGACATCTGAATTTATATACCTGTTGAGTTTTGAGTGCAC
  CCAAACACTCGATAAACCAGGTGAAGAAATTTAGCTTCCATGTTCTACTTCAGCTAAAACAGCTACATAC
  AACCTAGTACACTTGAAGTCAGACAGACATTTCAGTTGCTTACCTCCAGTACTGAGCCTTGCTTTGGGAA
  ACTAAAAGATTTAGACCAAGTCACTGCCAGTTTTTGCCTTTGTTGCATTTTGTACAGTTTTTATATTTTT
  GATATCTTGTAAATAAAGACAACCAGCTTTTCCAGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006951.3
• Summary: 'Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]'
• Locus ID: 6877