TAF11 (NM_005643) Human 3' UTR Clone

CAT#: SC210727

3`UTR clone of TAF11 RNA polymerase II TATA box binding protein (TBP)-associated factor 28kDa (TAF11) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TAF11
• Synonyms: MGC:15243; PRO2134; TAF2I; TAFII28
• ACCN: NM_005643
• Insert Size: 833 bp
• Sequence Data:
  Insert Sequence

  >SC210727 3'UTR clone of NM_005643
  The sequence shown below is from the reference sequence of NM_005643. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGACAGATCCCTAACTCGAAGCACAAAAAAATCATCTTCTTCTAGACCAAAGTCTAGAAAGGCCTATGT
  TACTGACGGAAGAAGTATTGGTTCCAGACTTCCTATAAGACTGTCTGCATTGGTGCTTTAGTATCTCAGG
  CCTCCAAGGATTCCATGATGATTTTAATGTCTTTCTCAAAACTCTGATATTTGTCACACCTAGAAAGTAT
  GTAGCCTGATTGATACTTGCCTTGACTAAATTTTGGGACCTCTTGGGGCATTTTGAAGTATTTAACTGTC
  TTGACCAGTTGGAAGAAGATACGTGGGCCATAAGCATCTTCTGGACAGGGGAACTGCTTTCAGAGAGAAA
  ACCTTTCCAAGAGAGTTTTGTTTTGTTTTGGTTTCGTTTTGTTTGAGATAGGGTCTTGCTCTATCACCTA
  GGCTGGAGTGCAGCGGCATGACTGCAGCCTTGAACTCCTGGGCTTAAGTGACCCTCCCACCTCAGTCTCC
  TGAGTAGCTAGGACTACAGGCACACACTACTGTGCCCAGCTAACTTATTTTTATTTTTTATGGAGATGGG
  GTCTTGCTTTGTTGCCCAGGCTGGTCGTGAACTCCTGGCTTCAAGCAGTCCTCCTGCCTCAGCCTCCTAA
  AGTGCCGAGGGCTTTAATGGTTTCACATTGAAGCCTGAAGTTGCTAAGACTTAGGTTGTTTCTTATATCT
  GGTTTTAAGTAGATGAAACAACCAGAAACTTTTACTTGTGATACTCTACCATGAAGGATGCGGTAATGGC
  AGGAATAGCAGAATAATTGGTGCTTGTAAACATTTAAGATTCTCCTGTGGATTTTGGTGAGTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005643.2
• Summary: 'Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a small subunit of TFIID that is present in all TFIID complexes and interacts with TBP. This subunit also interacts with another small subunit, TAF13, to form a heterodimer with a structure similar to the histone core structure. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]'
• Locus ID: 6882