Topoisomerase III alpha (TOP3A) (NM_004618) Human 3' UTR Clone

CAT#: SC210752

3`UTR clone of topoisomerase (DNA) III alpha (TOP3A) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TOP3A
• Synonyms: MGRISCE2; PEOB5; TOP3; ZGRF7
• ACCN: NM_004618
• Insert Size: 904 bp
• Sequence Data:
  Insert Sequence

  >SC210752 3'UTR clone of NM_004618
  The sequence shown below is from the reference sequence of NM_004618. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCCTTTTGTCCTCAGAACAGATGAGCTCAGGGTAGGGTAGAGAACGCCACTTTCTCAGACCTGTCCCCTT
  TGTGTTTAGAAATGAGTTAACCAGGACCAAGTGGCCATTTAGTGTCCTGGAAACTTAGAGGACAGTGTTG
  GCCTTTGGAGTCGGGCCTTCTTGTGTTAAGGGGCACAAGGTCCAGATCACTCTGGAGCAGGCCAGCTCTG
  CTGGACAGTGACCCTCTTCCCAGGCCTCAGGAGTGACCATAGCCACTGCTGAAAAGTCACGCAGCTGCTC
  CCTCGGACCCCCCAAGGATGGTTGCTGTTAGCAGAGGATTGGTGCAGTCCCAGCTGAAGCCCACTGTGTG
  CCAAAGGAAGAAGCTCCCAGGGCTGCTTCCTTCACCTGCAGAAAGCCCCAAGTGAGCCACCAGCACTCAT
  GGGGCAGTCCCTGTCCAGGCTGCCCAGGGCTTCTCATAGACGTCCTGAGAAGGACGGTGTAATGCAAGGA
  AATGGCTGTGGTAACACTGATCCTTCAGAAGAAGCTTCATTCCCTCTTAATCTAGTTAAGCCAGGACATC
  CAGAATTCATTGCTTTAATAAAGAACCCAGGCCGGGTGCAGTGGCTCATGCCTGTAATCCCAGCACTTTG
  GGAGGCTGTGGGGGGCGGATTGCCTGAGCTCAGGAGTTCGAGACCAGCCAGGGCAACATGGTGAAACCCT
  GTCTCTACTAAAATACAAAAAATTAGCCAGGTGTGGCGGTGTGCGCCTGTAGACCCGGCTACTCAGGAGG
  CTGAGGCAGGAGAATTGCTTGAACCCGGGAGGCGGAGGTTGCAGTTAGCTGAGATTGCGCCACTGGACGA
  CAGAGCGAGACTCAGTCTCAAAAAAAATTTAAAAATTTAAAAAAATAAAAAGAATCCAACCCCG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004618.3
• Summary: 'This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]'
• Locus ID: 7156