Glutathione Peroxidase 3 (GPX3) (NM_002084) Human 3' UTR Clone

CAT#: SC210760

3`UTR clone of glutathione peroxidase 3 (plasma) (GPX3) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GPX3
• Synonyms: GPx-P; GSHPx-3; GSHPx-P
• ACCN: NM_002084
• Insert Size: 900 bp
• Sequence Data:
  Insert Sequence

  >SC210760 3'UTR clone of NM_002084
  The sequence shown below is from the reference sequence of NM_002084. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGTCCTACATGAGGCGGCAGGCAGCCCTGGGGGTCAAGAGGAAGTAACTGAAGGCCGTCTCATCCCATGT
  CCACCATGTAGGGGAGGGACTTTGTTCAGGAAGAAATCCGTGTCTCCAACCACACTATCTACCCATCACA
  GACCCCTTTCCTATCACTCAAGGCCCCAGCCTGGCACAAATGGATGCATACAGTTCTGTGTACTGCCAGG
  CATGTGGGTGTGGGTGCATGTGGGTGTTTACACACATGCCTACAGGTATGCGTGATTGTGTGTGTGTGCA
  TGGGTGTACAGCCACGTGTCTACCTATGTGTCTTTCTGGGAATGTGTACCATCTGTGTGCCTGCAGCTGT
  GTAGTGCTGGACAGTGACAACCCTTTCTCTCCAGTTCTCCACTCCAATGATAATAGTTCACTTACACCTA
  AACCCAAAGGAAAAACCAGCTCTAGGTCCAATTGTTCTGCTCTAACTGATACCTCAACCTTGGGGCCAGC
  ATCTCCCACTGCCTCCAAATATTAGTAACTATGACTGACGTCCCCAGAAGTTTCTGGGTCTACCACACTC
  CCCAACCCCCCACTCCTACTTCCTGAAGGGCCCTCCCAAGGCTACATCCCCACCCCACAGTTCTCCCTGA
  GAGAGATCAACCTCCCTGAGATCAACCAAGGCAGATGTGACAGCAAGGGCCACGGACCCCATGGCAGGGG
  TGGCGTCTTCATGAGGGAGGGGCCCAAAGCCCTTGTGGGCGGACCTCCCCTGAGCCTGTCTGAGGGGCCA
  GCCCTTAGTGCATTCAGGCTAAGGCCCCTGGGCAGGGATGCCACCCCTGCTCCTTCGGAGGACGTGCCCT
  CACCCCTCACTGGTCCACTGGCTTGAGACTCACCCCGTCTGCCCAGTAAAAGCCTTTCTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002084.3
• Summary: 'The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is secreted, and is abundantly found in plasma. Downregulation of expression of this gene by promoter hypermethylation has been observed in a wide spectrum of human malignancies, including thyroid cancer, hepatocellular carcinoma and chronic myeloid leukemia. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016]'
• Locus ID: 2878