Carbonic Anhydrase III (CA3) (NM_005181) Human 3' UTR Clone

CAT#: SC210807

3`UTR clone of carbonic anhydrase III muscle specific (CA3) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CA3
• Synonyms: CAIII; Car3
• ACCN: NM_005181
• Insert Size: 893 bp
• Sequence Data:
  Insert Sequence

  >SC210807 3'UTR clone of NM_005181
  The sequence shown below is from the reference sequence of NM_005181. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TAACAGGGTGGTGAGAGCTTCCTTCAAATGAGGCTGCTGGATCTTGCCCTCTTCAGGAAAGGAAACCTAC
  CATTGGAGAGCTTGGTTCCTTGCCTCCTTCTGGTGCTCCTTACTCCAAGTCTATTTCATTTTTCCACACT
  GAGCAATGAATGTGAGAGATGTGGTCACCAAGATCTAAGTTACTTGTTGAAAGAAAGTTACTTTCGACAA
  GATCTAATATGAAAGCATAGATTTCACATTTGATCTCTGTAATAATCATCTTTCCTATAAAAGTAGCATT
  TTTGGTAAAGTTTCAAAGAAGAAGAAACAGAGATGGAAGAGTAAAGATATTTTTAAAATGGCTAGCTATT
  GGGCACCAGTTTTTCTGTTATCTAAAATTTCACACAACTTCATTGTTTTTATTTTTATATTATGAGTTGT
  CCATCTTAAAGAAATATGAGTAATTCTACATGTAGTAGAGGTGTATGAAGATCATATAACAATTAAACAT
  AAGCCAGAAATTAAAATGACTATAGACAGCAAGAATTGAGCTAATAATATGTTTTAACTCTTAACACCAG
  CAAGAAGTCAGTCATTTATTGAAGTTTTAGCTACTAAGATTACTTGGTTTTGATTACCAGTGAAAAGAAA
  ACACAATACAATCAGGAGTTTTCAAATTTTTGATTCAGTATTTGAATTTCTTCTTCATAAATGTAGTTGA
  ATTTATCCTAGTATTTTTCTTTACCTGAAGGAGGGCCATTTATTTTTAATTTCACTACATTTTTCTTTGC
  ATGATTATTAAAATAAAAACTGCCTCTGTTGTGTTTCTCACTGGAGGCTGGAATGAATGATCACTAGAAC
  ACAAAAGAGTGAATGATGACACTTGAAGTCAAAGCAGTTGTACTGATCACCAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005181.3
• Summary: 'Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008]'
• Locus ID: 761