BHMT2 (NM_017614) Human 3' UTR Clone

CAT#: SC210861

3`UTR clone of betaine-homocysteine methyltransferase 2 (BHMT2) for miRNA target validation


Reconstitution Protocol

USD 560.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol BHMT2
Synonyms FLJ20001
ACCN NM_017614
Insert Size 897
Sequence Data
>SC210861 3'UTR clone of NM_017614
The sequence shown below is from the reference sequence of NM_017614. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

TTCGCTGTCAAAGCCAGACTTCTAAGGAGTAGTGAAAGAAAACCCTGAAATAATCGAACAGGAAAAAGTT
GCCCTCAAGCCTGACCTGGAACCGTTCCTCACCTTCATCCTCACCATGCCCTGCTATCTCCAGCTGCTGA
GCAGCTGAGGTGCTGCAGCCCCTTCCCTTCCAGCCCACAAGTGTGTGCATATTGAGCTCCTGCTGTGGTT
AAGCACTGCAACAGACTCTACCAGAGATGCAAAGAGAAGCGAGAGAGGCACCTTGTTCTCCAAGAACTTA
CTGTCCAATCATGCTGTGGTGCATTCCTTTGAAGATCATGAAGAATAGCCAAACTTGTCTTTGAGGGTGA
ATTTGACACTTTAAAATAATCAGAAGTCACTGAGACCCAAAGCTAGTGAATAATTCAAGTGACTAAGCTA
GATACTTGAGGATGATAGAGCAACACTAAACTAAAGCAATGAGTCTGCTTTTTTTAATGATTTATACAAT
GACTCCAAAGGCAATCCCAAAGAAAAGATGTAAAGAATGTTTTGAGCAATGGTAGCATTATTGAAATAAA
TGTTTAACTATCCAAAGTGATTACTTTCAAAGATGATTTTTACTTAAAGATATTAGGTCTGGCCAGGCGA
AGTGACTCACACCTGCAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTCAGCAGTTC
AAGACCAGCCTGACCAACATGGTGAAACCCTGTCTCTACCAAAATACAAAAATTAGCCGGGCATGATGGC
GGGTGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCGGAGCTT
GCAGTGAGCCGAGATCAGGCCATTGCACTTCAGCCTGGGTGACAGAGTGAGACTCCA

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq NM_017614.3
Summary Homocysteine is a sulfur-containing amino acid that plays a crucial role in methylation reactions. Transfer of the methyl group from betaine to homocysteine creates methionine, which donates the methyl group to methylate DNA, proteins, lipids, and other intracellular metabolites. The protein encoded by this gene is one of two methyl transferases that can catalyze the transfer of the methyl group from betaine to homocysteine. Anomalies in homocysteine metabolism have been implicated in disorders ranging from vascular disease to neural tube birth defects such as spina bifida. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]
Locus ID 23743

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.