Adenosine Receptor A2a (ADORA2A) (NM_000675) Human 3' UTR Clone

CAT#: SC210862

3`UTR clone of adenosine A2a receptor (ADORA2A) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ADORA2A
• Synonyms: A2aR; ADORA2; RDC8
• ACCN: NM_000675
• Insert Size: 873 bp
• Sequence Data:
  Insert Sequence

  >SC210862 3'UTR clone of NM_000675
  The sequence shown below is from the reference sequence of NM_000675. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGATGGAGCAGGAGTGTCCTGATGATTCATGGAGTTTGCCCCTTCCTAAGGGAAGGAGATCTTTATCTT
  TCTGGTTGGCTTGACCAGTCACGTTGGGAGAAGAGAGAGAGTGCCAGGAGACCCTGAGGGCAGCCGGTTC
  CTACTTTGGACTGAGAGAAGGGAGCCCCAGGCTGGAGCAGCATGAGGCCCAGCAAGAAGGGCTTGGGTTC
  TGAGGAAGCAGATGTTTCATGCTGTGAGGCCTTGCACCAGGTGGGGGCCACAGCACCAGCAGCATCTTTG
  CTGGGCAGGGCCCAGCCCTCCACTGCAGAAGCATCTGGAAGCACCACCTTGTCTCCACAGAGCAGCTTGG
  GCACAGCAGACTGGCCTGGCCCTGAGACTGGGGAGTGGCTCCAACAGCCTCCTGCCACCCACACACCACT
  CTCCCTAGACTCTCCTAGGGTTCAGGAGCTGCTGGGCCCAGAGGTGACATTTGACTTTTTTCCAGGAAAA
  ATGTAAGTGTGAGGAAACCCTTTTTATTTTATTACCTTTCACTCTCTGGCTGCTGGGTCTGCCGTCGGTC
  CTGCTGCTAACCTGGCACCAGAGCCTCTGCCCGGGGAGCCTCAGGCAGTCCTCTCCTGCTGTCACAGCTG
  CCATCCACTTCTCAGTCCCAGGGCCATCTCTTGGAGTGACAAAGCTGGGATCAAGGACAGGGAGTTGTAA
  CAGAGCAGTGCCAGAGCATGGGCCCAGGTCCCAGGGGAGAGGTTGGGGCTGGCAGGCCACTGGCATGTGC
  TGAGTAGCGCAGAGCTACCCAGTGAGAGGCCTTGTCTAACTGCCTTTCCTTCTAAAGGGAATGTTTTTTT
  CTGAGATAAAATAAAAACGAGCCACATCGTGTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000675.4
• Summary: 'This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013]'
• Locus ID: 135