RAF1 (NM_002880) Human 3' UTR Clone

CAT#: SC211172

3`UTR clone of v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RAF1
• Synonyms: c-Raf; CMD1NN; CRAF; NS5; Raf-1
• ACCN: NM_002880
• Insert Size: 929 bp
• Sequence Data:
  Insert Sequence

  >SC211172 3'UTR clone of NM_002880
  The sequence shown below is from the reference sequence of NM_002880. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAATGCTTGCACGCTGACCACGTCCCCGAGGCTGCCTGTCTTCTAGTTGACTTTGCACCTGTCTTCAGGC
  TGCCAGGGGAGGAGGAGAAGCCAGCAGGCACCACTTTTCTGCTCCCTTTCTCCAGAGGCAGAACACATGT
  TTTCAGAGAAGCTGCTGCTAAGGACCTTCTAGACTGCTCACAGGGCCTTAACTTCATGTTGCCTTCTTTT
  CTATCCCTTTGGGCCCTGGGAGAAGGAAGCCATTTGCAGTGCTGGTGTGTCCTGCTCCCTCCCCACATTC
  CCCATGCTCAAGGCCCAGCCTTCTGTAGATGCGCAAGTGGATGTTGATGGTAGTACAAAAAGCAGGGGCC
  CAGCCCCAGCTGTTGGCTACATGAGTATTTAGAGGAAGTAAGGTAGCAGGCAGTCCAGCCCTGATGTGGA
  GACACATGGGATTTTGGAAATCAGCTTCTGGAGGAATGCATGTCACAGGCGGGACTTTCTTCAGAGAGTG
  GTGCAGCGCCAGACATTTTGCACATAAGGCACCAAACAGCCCAGGACTGCCGAGACTCTGGCCGCCCGAA
  GGAGCCTGCTTTGGTACTATGGAACTTTTCTTAGGGGACACGTCCTCCTTTCACAGCTTCTAAGGTGTCC
  AGTGCATTGGGATGGTTTTCCAGGCAAGGCACTCGGCCAATCCGCATCTCAGCCCTCTCAGGGAGCAGTC
  TTCCATCATGCTGAATTTTGTCTTCCAGGAGCTGCCCCTATGGGGCGGGGCCGCAGGGCCAGCCTTGTTT
  CTCTAACAAACAAACAAACAAACAGCCTTGTTTCTCTAGTCACATCATGTGTATACAAGGAAGCCAGGAA
  TACAGGTTTTCTTGATGATTTGGGTTTTAATTTTGTTTTTATTGCACCTGACAAAATACAGTTATCTGAT
  GGTCCCTCAATTATGTTAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002880.3
• Summary: 'This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]'
• Locus ID: 5894