POLE (NM_006231) Human 3' UTR Clone

CAT#: SC211334

3`UTR clone of polymerase (DNA directed) epsilon (POLE) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: POLE
• Synonyms: CRCS12; FILS; IMAGEI; POLE1
• ACCN: NM_006231
• Insert Size: 934 bp
• Sequence Data:
  Insert Sequence

  >SC211334 3'UTR clone of NM_006231
  The sequence shown below is from the reference sequence of NM_006231. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCAGAAGAACCCACAGCTGGGCCATTAGCCAGCCCCGGGCCCCGGGTGCCTCTGCGTCCGTGCCAGGCCT
  CCTGATGCCAAGGCCACATCCCCGTGCTTCCAGTGACCAGACCACTGACCACCCTGACTGTCCAAACCTG
  TGACCCCAGGCCAGGGAACGGGGAGGAAACCAAAGAAAACCATTTTCAGGGAGCTCAGACGTCACAGGAG
  GGAGCGGGAGCAGGATGTGGCCCTGGCCTCGCCAGAGCACCTGAAGAAGCAGGCCGTGAGCGAGGCTGCG
  AGTGCCCTGGGCGCCGTTTCTCACGCAGTGAATGCTTTTCCAGGCCTCTGTTGCTTCCTGCACCACACCT
  GGTGGGGTGGGAGCGTCCTCTAGGTGCCCCTAGTTCTTTGTCCTGCCTCCCAGAGGGAGGAAAAGCCCCT
  GGGGGCTTCTGGCTCCCTGAGATTGGGCTCTGAGACGAGACGGGTTCCCAAGGCCCTGGTGGGGCTGGAG
  TCTCACCTGTTTGCATGGAGAAATGGGCTGGCCCCACAGCCTCACAGGAGCAGTTTGTGGGCTGGTTTCC
  CCAGGAATCCAGACCCTAACCCGTGAGAATCTGGATTTTGGCTTGTGAGCCCTGCTTATTTGGAGCCGGG
  TCTAGAGGGAACCCTCTATCAGCCTCAGGAAAACAAGACCTCTGTGCACCTCACTTTTGGCTCACTGCAG
  CCCTTGTCCTTCACCTCCACACAGGACCAGCTGGAAGCAGAAAGAAGAAAGGCCAATTTCACAGGGCACC
  AAACAAGTATGAAATGTAAATCAGAAATGCAGACACCCCAGACGAGAGCCTCACAGGAGGGAGGGGGCCC
  CACAGGCTCCCCAGGAGGCTCGTGTCTTTGGCCCAGAGCCAGCCTTAGTTTGTCCCTGCCATCTACTGTC
  TGAGGCCATCGCTGCTACACTTTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006231.2
• Summary: 'This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013]'
• Locus ID: 5426