FGFR1 (NM_023108) Human 3' UTR Clone

CAT#: SC211346

3`UTR clone of fibroblast growth factor receptor 1 (FGFR1) transcript variant 6 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: FGFR1
• Synonyms: BFGFR; CD331; CEK; FGFBR; FLG; FLJ99988; FLT2; HBGFR; KAL2; N-SAM; OGD
• ACCN: NM_023108
• Insert Size: 962 bp
• Sequence Data:
  Insert Sequence

  >SC211346 3'UTR clone of NM_023108
  The sequence shown below is from the reference sequence of NM_023108. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGGGAACAGAGGGCTGCAGGGATGGGTGGTGCTGGTCTGTAATAAGCTTTGAGAGCAACGTCACTGGGGC
  TTTGGGGTCAGCTACACAAGGAAGGCATTTGGACCCCTGCCTTTTCATTGCCCGAAACCAGAGCCTTTCC
  ACCAAGCGTTTCCCAGTCTTAGCCCTGTGTCCTGAGTTACGTACGATCTTTCTGGCAAATGGGGTGCATG
  ATAAGAGCATCTCTTACGAAGAGTTGGAAAAACAAATGCCATATATAAATTCTAAGCCATATGAGGACGA
  GGAGTAATGGCATTTTCTTCCTTTTTCCTCTCACTCCCAGACATTCATTGTCCCTGAATGCTCCATTAAT
  CCAGGGAAGGTAATTGCCTAAATCTCCAGTGGATCTCGCAACAGGAAGGAACCAGAAGCTGGGAAAGTTG
  TTTACCTCTTTGTCCCAGAGTTAGACCTCATCCTCCCCTAGCTTAGCTGTCTCAGAGATATACTGGCCCT
  CCCTTCTCTTCTCTTTGCTGCTGGTGCTAAAACTGCTCTGTAGGTCATTGGCCACTGTCTCCACTCACAA
  CCCCTGCTCCAGTCCTGGAGGGAGTGGGTTAAACACAAATAGAACATTCCATTTGAAGCAGTGATTCTTT
  TTTTTTTTTTTTTTTTTTAATCAAATGCTTTGGACTTTTGAAGTCCACTTGTTCTGTACTTGTAAAAGGG
  AAAGAAGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGCACTTTAGATCACTTGAGGTCAGGAGTTTG
  AGACCAGCCCGGCCAACATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGTGCATAGTGGT
  TGGCACCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAAGCTAACTGCTTGAACCCAGAAGGCAGAGGTT
  GCAGTGAGCTGAGATCACGCCACTGCACTCCAGCCTGGGTGACAGAGTGAGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_023108.2
• Summary: 'The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]'
• Locus ID: 2260