RBM3 (NM_006743) Human 3' UTR Clone

CAT#: SC211487

3`UTR clone of RNA binding motif (RNP1 RRM) protein 3 (RBM3) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RBM3
• Synonyms: IS1-RNPL; RNPL
• ACCN: NM_006743
• Insert Size: 1015 bp
• Sequence Data:
  Insert Sequence

  >SC211487 3'UTR clone of NM_006743
  The sequence shown below is from the reference sequence of NM_006743. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATGACCGCTACTCAGGAGGAAATTACAGAGACAATTATGACAACTGAAATGAGACATGCACATAATATAG
  ATACACAAGGAATAATTTCTGATCCAGGATCGTCCTTCCAAATGGCTGTATTTATAAAGGTTTTTGGAGC
  TGCACCGAAGCATCTTATTTTATAGTATATCAACCTTTTGTTTTTAAATTGACCTGCCAAGGTAGCTGAA
  GACCTTTTAGACAGTTCCATCTTTTTTTTTAAATTTTTTCTGCCTATTTAAAGACAAATTATGGGACGTT
  TGTAGAACCTGAGTATTTTTCTTTTTACCAGTTTTTTAGTTTGAGCTCTTAGGTTTATTGGAGCTAGCAA
  TAATTGGTTCTGGCAAGTTTGGCCAGACTGACTTCAAAAAATTAATGTGTATCCAGGGACATTTTAAAAA
  CCTGTACACAGTGTTTATTGTGGTTAGGAAGCAATTTCCCAATGTACCTATAAGAAATGTGCATCAAGCC
  AGCCTGACCAACATGGTGAAACCCCATCTGTACTAAACATAAAAAAATTAGCCTGGCATGGTGGTGTACG
  CCTGTAATCCCAGTGACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGT
  GAGCTAAGATCGCGCCACTGTACTCCAGCCTGGGCAACAGCGAGACTCCATCTCAAAAAAAAAGGAAATG
  TGTATCAAGAACATGATTATCCAGCGGTATTTTCTAATTCAGATCATCAAACTGATTATATAGAAGAGTT
  GGCTTTAAAATGTTTGCAAATGTCTTTTTTTTTTTAATACTGGAAGAAAAAATATTCTGTTGTGTCTCAT
  ACAGTGCTTAGGATGTCTTTCACAGAGCTTATTAAAAAGATGAAACCTGAGAACAAACTGCTTTATTCTT
  ACTCAGCCCATTTTGCAAATTAAAAGTGGGGGCAGAGGTGGGCGGATCACCTGAGGTCAGGAGTTCGAGA
  CCAGCCTGGCCAACAGGGCAAAACCCCATCTCTAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006743.3
• Summary: 'This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2008]'
• Locus ID: 5935