CD40L (CD40LG) (NM_000074) Human 3' UTR Clone

CAT#: SC211497

3`UTR clone of CD40 ligand (CD40LG) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CD40LG
• Synonyms: CD40L; CD154; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP
• ACCN: NM_000074
• Insert Size: 1010 bp
• Sequence Data:
  Insert Sequence

  >SC211497 3'UTR clone of NM_000074
  The sequence shown below is from the reference sequence of NM_000074. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACTGGCTTCACGTCCTTTGGCTTACTCAAACTCTGAACAGTGTCACCTTGCAGGCTGTGGTGGAGCTGAC
  GCTGGGAGTCTTCATAATACAGCACAGCGGTTAAGCCCACCCCCTGTTAACTGCCTATTTATAACCCTAG
  GATCCTCCTTATGGAGAACTATTTATTATACACTCCAAGGCATGTAGAACTGTAATAAGTGAATTACAGG
  TCACATGAAACCAAAACGGGCCCTGCTCCATAAGAGCTTATATATCTGAAGCAGCAACCCCACTGATGCA
  GACATCCAGAGAGTCCTATGAAAAGACAAGGCCATTATGCACAGGTTGAATTCTGAGTAAACAGCAGATA
  ACTTGCCAAGTTCAGTTTTGTTTCTTTGCGTGCAGTGTCTTTCCATGGATAATGCATTTGATTTATCAGT
  GAAGATGCAGAAGGGAAATGGGGAGCCTCAGCTCACATTCAGTTATGGTTGACTCTGGGTTCCTATGGCC
  TTGTTGGAGGGGGCCAGGCTCTAGAACGTCTAACACAGTGGAGAACCGAAACCCCCCCCCCCCCCCCGCC
  ACCCTCTCGGACAGTTATTCATTCTCTTTCAATCTCTCTCTCTCCATCTCTCTCTTTCAGTCTCTCTCTC
  TCAACCTCTTTCTTCCAATCTCTCTTTCTCAATCTCTCTGTTTCCCTTTGTCAGTCTCTTCCCTCCCCCA
  GTCTCTCTTCTCAATCCCCCTTTCTAACACACACACACACACACACACACACACACACACACACACACAC
  ACACACACAGAGTCAGGCCGTTGCTAGTCAGTTCTCTTCTTTCCACCCTGTCCCTATCTCTACCACTATA
  GATGAGGGTGAGGAGTAGGGAGTGCAGCCCTGAGCCTGCCCACTCCTCATTACGAAATGACTGTATTTAA
  AGGAAATCTATTGTATCTACCTGCAGTCTCCATTGTTTCCAGAGTGAACTTGTAATTATCTTGTTATTTA
  TTTTTTGAATAATAAAGACCTCTTAACATT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000074.2
• Summary: 'The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]'
• Locus ID: 959