B4GALNT1 (NM_001478) Human 3' UTR Clone

CAT#: SC211507

3`UTR clone of beta-14-N-acetyl-galactosaminyl transferase 1 (B4GALNT1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: B4GALNT1
• Synonyms: GALGT; GalNAc-T; GALNACT; SPG26
• ACCN: NM_001478
• Insert Size: 1009 bp
• Sequence Data:
  Insert Sequence

  >SC211507 3'UTR clone of NM_001478
  The sequence shown below is from the reference sequence of NM_001478. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGCTCTTCTTCAAACACCGGCTGCAGTGCATGACCTCCCAGTGATGGCCCGCTGGGGATTTCTGACTGT
  CAGGCTGGGCCTGCCTCCTTGTCCCTGCCAGGAATTTCCAACAAACCCCACCACCCTGTGAGCACTCTAC
  TGGCTGTCCCTGAGCCTCTAGTTCCTCACTCTTCCTTTTCAGAACCTGATGCCCAGTAGGGGTTGTCCTG
  GTGACACCCCTCCTTTTTCCAGTGCCCAGAGGCCTGGTGGAGCCATAACCTCTCCCACAGCCAGTGCCAA
  GTCCTCCCCCTGCCCATTCTCATGGGGCAGGAAATGGGGGGATCACTTTCCAAGTGCCAAAGAGCCCAGA
  GGGACTCTAAGAACCTAAGGTGGAAACACTGTCCTCTCATCTTGGGACCGAGGGGGTGGGGAAGTTCCCC
  AACACATAATCCCAAGACTGTGCCCCTCATCTGCATCTTCAGATCCAGTACTCTGTGTACCTGCTCCAGC
  CCCACCCCCACAGAGAGAACTTGTGGCTCTGGGGCTGGGGTGAGGGCTGGTGGTTGGTGAAAGCCATTCT
  TAGTTGTGTCTCTGCAATGCTGTGGGCACAAAAGAAGGGGCACCAGAGTCCCTGTGCAAACACCTAGACT
  CACTTCATGGATTCCAAAGCTCTCAGCTTCATTTTATTAGTTACGTTAGGTAAGGGGGTTCAAGGGTCAT
  GGTCCTCATCACACACATGTCATCAGGGCCCTCTGCACTCCACATGATGAGGTCAGACCCACACGGTGCA
  AATCTTTGGGTCAGTGAGCTCCTGGAGAAGAGAGGAGACATGTCAGGAATAGATTAGGCACCCCTCTTCC
  TTAATGAAATGTGGCAGTCCTCTCAGGGGTACCCCACCTACTTAGGGATCTGAGTTCTTCAGTTCCTCAC
  CCATAGCTCAGCCCGGGCTCCCTGATTTGGTCTTCAATCTACTATTCATTCCGTCCCCACCCTTTTCTTC
  TTGATCTTCCTCAGACCCTTTCCCAAACC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001478.3
• Summary: 'GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]'
• Locus ID: 2583