ATP citrate lyase (ACLY) (NM_001096) Human 3' UTR Clone

CAT#: SC211547

3`UTR clone of ATP citrate lyase (ACLY) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ACLY
• Synonyms: ACL; ATPCL; CLATP
• ACCN: NM_001096
• Insert Size: 881 bp
• Sequence Data:
  Insert Sequence

  >SC211547 3'UTR clone of NM_001096
  The sequence shown below is from the reference sequence of NM_001096. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCGGAACACATGAGCATGTAACAGAGCCAGGAACCCTACTGCAGTAAACTGAAGACAAGATCTCTTCCCC
  CAAGAAAAAGTGTACAGACAGCTGGCAGTGGAGCCTGCTTTATTTAGCAGGGGCCTGGAATGTAAACAGC
  CACTGGGGTACAGGCACCGAAGACCAACATCCACAGGCTAACACCCCTTCAGTCCACACAAAGAAGCTTC
  ATATTTTTTTTATAAGCATAGAAATAAAAACCAAGCCAATATTTGTGACTTTGCTCTGCTACCTGCTGTA
  TTTATTATATGGAAGCATCTAAGTACTGTCAGGATGGGGTCTTCCTCATTGTAGGGCGTTAGGATGTTGC
  TTTCTTTTTCCATTAGTTAAACATTTTTTTCTCCTTTGGAGGAAGGGAATGAAACATTTATGGCCTCAAG
  ATACTATACATTTAAAGCACCCCAATGTCTCTCTTTTTTTTTTTTTACTTCCCTTTCTTCTTCCTTATAT
  AACATGAAGAACATTGTATTAATCTGATTTTTAAAGATCTTTTTGTATGTTACGTGTTAAGGGCTTGTTT
  GGTATCCCACTGAAATGTTCTGTGTTGCAGACCAGAGTCTGTTTATGTCAGGGGGATGGGGCCATTGCAT
  CCTTAGCCATTGTCACAAAATATGTGGAGTAGTAACTTAATATGTAAAGTTGTAACATACATACATTTAA
  AATGGAAATGCAGAAAGCTGTGAAATGTCTTGTGTCTTATGTTCTCTGTATTTATGCAGCTGATTTGTCT
  GTCTGTAACTGAAGTGTGGGTCCAAGGACTCCTAACTACTTTGCATCTGTAATCCACAAAGATTCTGGGC
  AGCTGCCACCTCAGTCTCTTCTCTGTATTATCATAGTCTGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001096.2
• Summary: 'ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]'
• Locus ID: 47