PLC delta 3 (PLCD3) (NM_133373) Human 3' UTR Clone

CAT#: SC211637

3`UTR clone of phospholipase C delta 3 (PLCD3) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PLCD3
• Synonyms: PLC-delta-3
• ACCN: NM_133373
• Insert Size: 1007
• Sequence Data:
  Insert Sequence

  >SC211637 3'UTR clone of NM_133373
  The sequence shown below is from the reference sequence of NM_133373. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CGCTCTTCATCCAAATCCGCATCCAGCGCTCCTGAGGGCCCACCTCACTCGCCTTGGGGTTCTGCGAGTG
  CCAGTCCACATCCCCTGCAGAGCCCTCTCCTCCTCTGGAGTCAGGTGGTGGGAGTACCAGCCCCCCAGCC
  CACCCACTTGGCCCACTCAGCCCATTCACCAGGCGCTGGTCTCACCTGGGTGCTGAGGGCTGCCTGGGCC
  CCTCCTGAAGAACAGAAAGGTGTTCATGTGACTTCAGTGAGCTCCAACCCTGGGGCCCTGAGATGGCCCC
  AGCTCCTCTTGTCCTCAGCCCACCCCTCATTGTGACTTATGAGGAGCAAGCCTGTTGCTGCCAGGAGACT
  TGGGGAGCAGGACACTTGTGGGCCCTCAGTTCCCCTCTGTCCTCCCGTGGGCCATCCCAGCCTCCTTCCC
  CCAGAGGAGCGCAGTCACTCCACTTGGCCCCGACCCCGAGCTTAGCCCCTAAGCCCTCCTTTACCCCAGG
  CCTTCCTGGACTCCTCCCTCCAGCTCCGGAACCTGAGCTCCCCTTCCCTTCTCAAAGCAAGAAGGGAGCG
  CTGAGGCATGAAGCCCTGGGGAAACTGGCAGTAGGTTTTGGTTTTTATTTTTTGAGACAGGGTCTCGCTC
  CGTCGCCCAGGCTGGAGTGCAATGTTGCAATCATGGCTCACTGCAGCTTTGAACTCCCAGGCTCAAGCGA
  TCCTCCCATCTCAGCCTCCTGAGTAGCTGGGACTACAGGCACAGGCCACCAAACCTGGCTAATGTTTAAA
  TTTTATGTAGAGATGGCGGGGGGGGCGGGGGTCTCCCTATGTTGCCCAGGCTGGTCTCGAGCTCCTGACC
  TCAAGTGATCCTCCTGCCCCAGCATTCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCCTGGCCTGGC
  CGTAGGTTTGTAACTGTTTCATAGAAGAGCCCTGGAGAAGACAGTAGAATGAGCCTATCTAGTTTAAAAA
  ATAAAGAAGCACGTTGATTTCACCACC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_133373.3
• Summary: This gene encodes a member of the phospholipase C family, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate the second messengers diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Diacylglycerol and IP3 mediate a variety of cellular responses to extracellular stimuli by inducing protein kinase C and increasing cytosolic Ca(2+) concentrations. This enzyme localizes to the plasma membrane and requires calcium for activation. Its activity is inhibited by spermine, sphingosine, and several phospholipids. [provided by RefSeq, Jul 2008]
• Locus ID: 113026