NODAL (NM_018055) Human 3' UTR Clone

CAT#: SC211715

3`UTR clone of nodal homolog (mouse) (NODAL) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NODAL
• Synonyms: HTX5
• ACCN: NM_018055
• Insert Size: 1009 bp
• Sequence Data:
  Insert Sequence

  >SC211715 3'UTR clone of NM_018055
  The sequence shown below is from the reference sequence of NM_018055. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGAATGTGGGTGCCTCTGATGACATCCTGGAGGGAGACTGGATTTGCCTGCACTCTGGAAGGCTGGGAAA
  CTCCTGGAAGACATGATAACCATCTAATCCAGTAAGGAGAAACAGAGAGGGGCAAAGTTGCTCTGCCCAC
  CAGAACTGAAGAGGAGGGGCTGCCCACTCTGTAAATGAAGGGCTCAGTGGAGTCTGGCCAAGCACAGAGG
  CTGCTGTCAGGAAGAGGGAGGAAGAAGCCTGTGCAGGGGGCTGGCTGGATGTTCTCTTTACTGAAAAGAC
  AGTGGCAAGGAAAAGCACAAGTGCATGAGTTCTTTACTGGATTTTTTAAAAACCTGTGAACCCCCCGAAA
  CTGTATGTGAAAGTTGAGACATATGTGCATGTATTTTGGAGGTGGGATGAAGTCACCTATAGCTTTCATG
  TATTCTCCAAAGTAGTCTGTGTGTGACCTGTCCCCCTCCCCAAAGATTAAGGATCACTGTATAGATTAAA
  AAGAGTCCGTCAATCTCATTGCCTCAGGCTGGGTTGGGGGAGCCCCACAGCTTTCTGGCTGGCCAGTGGC
  AATCTACTGGCCTTGTCCAGAGGCTCACTGGAGTGGTTCTCTGCTAATGAGCTGTACAACAATAAAGCCA
  TTGTCTAGTTCTCCTGGGCCAGCTGGTGCCTGTGAAGGCAGAGGCAGGAACTCATCCAAGAGGACCGGCC
  ATGTTGGGTTACAGAAGACATCCCTGCGTCAGTCTGCTTCGGCAGACACAGCCTGAGTTTGTTAAAGTTG
  GTGACAATCCACCTCAGTCTCTCAATGTGTGCTATTAATGAGGCCTCTGAGCTTCCTATCCAGCAGTGGT
  GAAGGCCTTGCCCTGGGTGGCAAGATACTTGCTCTATGGTCACAGCTCAGCCACTGGAAGCTGTGCGACC
  TCAGGTGAGCAATTCACTGTCCAGTCTCCACTTGTAAAAGGAACGCTGGTGAATCCTAATGCATTCATAT
  TAAATGTCTGTTGTCAGGCTCAGAAGAGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_018055.4
• Summary: 'This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which regulates early embryonic development. This protein is required for maintenance of human embryonic stem cell pluripotency and may play a role in human placental development. Mutations in this gene are associated with heterotaxy, a condition characterized by random orientation of visceral organs with respect to the left-right axis. [provided by RefSeq, Aug 2016]'
• Locus ID: 4838