TIE2 (TEK) (NM_000459) Human 3' UTR Clone

CAT#: SC211718

3`UTR clone of TEK tyrosine kinase endothelial (TEK) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TEK
• Synonyms: CD202B; GLC3E; TIE-2; TIE2; VMCM; VMCM1
• ACCN: NM_000459
• Insert Size: 964 bp
• Sequence Data:
  Insert Sequence

  >SC211718 3'UTR clone of NM_000459
  The sequence shown below is from the reference sequence of NM_000459. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGTTCTGCTGAAGAAGCGGCCTAGGACAGAACATCTGTATACCCTCTGTTTCCCTTTCACTGGCATGGG
  AGACCCTTGACACCTGCTGAGAAAACATGCCTCTGCCAAAGGATGTGATATATAAGTGTACATATGTGCT
  GTACACCTGGGACCTTCACCACTGTAGATCCCATGCATGGATCTATGTAGTATGCTCTGACTCTAATAGG
  ACTGTATATACTGTTTTAAGAATGGGCTGAAATCAGAATGCCTGTTTGTGGTTTCATATGCAATAATATA
  TTTTTTTAAAAATGTGGACTTCATAGGAAGGCGTGAGTACAATTAGTATAATGCATAACTCATTGTTGTC
  CTAGATATTTTGATATTTACCTTTATGTTGAATGCTATTAAATGTTTTCCTGTGTCAAAGTAAAATATTG
  TTAATAAACCTAACAATGACCCTGATAGTACAGGTTAAGTGAGAGAACTATATGAATTCTAACAAGTCAT
  AGGTTAATATTTAAGACACTGAAAAATCTAAGTGATATAAATCAGATTCTTCTCTCTCAATTTTATCCCT
  CACCTGTAGCAGCCAGTCCCGTTTCATTTAGTCATGTGACCACTCTGTCTTGTGTTTCCACAGCCTGCAA
  GTCAGTCCAGGATGCTAACATCTAAAAATAGACTTAAATCTCATTGCTTACAAGCCTAAGAATCTTTAGA
  GAAGTATACATAAGTTTAGGATAAAATAATGGGATTTTCTTTTCTTTTCTCTGGTAATATTGACTTGTAT
  ATTTTAAGAAATAACAGAAAGCCTGGGTGACATTTGGGAGACATGTGACATTTATATATTGAATTAATAT
  CCCTACATGTATTGCACATTGTAAAAAGTTTTAGTTTTGATGAGTTGTGAGTTTACCTTGTATACTGTAG
  GCACACTTTGCACTGATATATCATGAGTGAATAAATGTCTTGCCTACTCACGTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000459.3
• Summary: 'This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]'
• Locus ID: 7010