PGK1 (NM_000291) Human 3' UTR Clone

CAT#: SC211796

3`UTR clone of phosphoglycerate kinase 1 (PGK1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PGK1
• Synonyms: HEL-S-68p; MIG10; PGKA
• ACCN: NM_000291
• Insert Size: 1018 bp
• Sequence Data:
  Insert Sequence

  >SC211796 3'UTR clone of NM_000291
  The sequence shown below is from the reference sequence of NM_000291. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGTAAAGTCCTTCCTGGGGTGGATGCTCTCAGCAATATTTAGTACTTTCCTGCCTTTTAGTTCCTGTGC
  ACAGCCCCTAAGTCAACTTAGCATTTTCTGCATCTCCACTTGGCATTAGCTAAAACCTTCCATGTCAAGA
  TTCAGCTAGTGGCCAAGAGATGCAGTGCCAGGAACCCTTAAACAGTTGCACAGCATCTCAGCTCATCTTC
  ACTGCACCCTGGATTTGCATACATTCTTCAAGATCCCATTTGAATTTTTTAGTGACTAAACCATTGTGCA
  TTCTAGAGTGCATATATTTATATTTTGCCTGTTAAAAAGAAAGTGAGCAGTGTTAGCTTAGTTCTCTTTT
  GATGTAGGTTATTATGATTAGCTTTGTCACTGTTTCACTACTCAGCATGGAAACAAGATGAAATTCCATT
  TGTAGGTAGTGAGACAAAATTGATGATCCATTAAGTAAACAATAAAAGTGTCCATTGAAACCGTGATTTT
  TTTTTTTTTCCTGTCATACTTTGTTAGGAAGGGTGAGAATAGAATCTTGAGGAACGGATCAGATGTCTAT
  ATTGCTGAATGCAAGAAGTGGGGCAGCAGCAGTGGAGAGATGGGACAATTAGATAAATGTCCATTCTTTA
  TCAAGGGCCTACTTTATGGCAGACATTGTGCTAGTGCTTTTATTCTAACTTTTATTTTTATCAGTTACAC
  ATGATCATAATTTAAAAAGTCAAGGCTTATAACAAAAAAGCCCCAGCCCATTCCTCCCATTCAAGATTCC
  CACTCCCCAGAGGTGACCACTTTCAACTCTTGAGTTTTTCAGGTATATACCTCCATGTTTCTAAGTAATA
  TGCTTATATTGTTCACTTCTTTTTTTTTTATTTTTTAAAGAAATCTATTTCATACCATGGAGGAAGGCTC
  TGTTCCACATATATTTCCACTTCTTCATTCTCTCGGTATAGTTTTGTCACAATTATAGATTAGATCAAAA
  GTCTACATAACTAATACAGCTGAGCTATGTAGTATGCT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000291.3
• Summary: 'The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]'
• Locus ID: 5230