RYK (NM_001005861) Human 3' UTR Clone

CAT#: SC211849

3`UTR clone of RYK receptor-like tyrosine kinase (RYK) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RYK
• Synonyms: D3S3195; JTK5; JTK5A; RYK1
• ACCN: NM_001005861
• Insert Size: 1056 bp
• Sequence Data:
  Insert Sequence

  >SC211849 3'UTR clone of NM_001005861
  The sequence shown below is from the reference sequence of NM_001005861. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAGTGCCTAACAGAGTTTCATGCAGCCCTGGGGGCCTACGTCTGACTCCTCTCCAATCCCACACCATCAG
  GAAGAAGGTGCCTGTCGGGGCTCACTTGAAGCCTGTCAGGGATGCTTTGTATCTAACACAACGCCAACAG
  AAGCACATTTGTCTTCCAGAACACCGTGCCTTAGAAATGCTTTAGAATCTGAACTTTTTAAGACAGACTT
  AATAATGTGGCATATTTTCTAGATATCACTTTTATTAGGTTGAACTGAAAGGGTTTTTGTAAATTTTTTG
  GCCAAAATTTTTTAAAACATACTTACTTTGGACTAGGGGTACATTCTTACAAAATAAATAAACAGTTTTT
  AAAATTGTTTAGACACAGATATTTGGAATTAGCTATCTTAGTGCCAACTGCTTTTTATTTTTTTACTTCA
  TCAAGGTGATGTAAGTGACTCACCTTTAAAGTTTTTTTAGTGTTATTTTTTATCACTACTCTGGGAAATG
  GTTTGTCTTCAAGATGCAATACTTTTCTTAGTAAAGGAAAAACAGCATAAAAAGATACCTGGTCTGCCTT
  GTACAAGAAAAGGCAATATTAGAGGAAGAAAATTTAAAGAAAAGCTAGAGGAAAAAAAAATTTTTTTAAA
  AATACTTATTAGAAGCAAACTGCCCTTGCATGGAAAACTGTTTATTTTTTTCAGTGAAAAGGAATTCTGC
  TTTCGTGTTTTTGGGAAAGCAGGAACTGAGTTCATTACATCTTTAATTTGGCAGAAATTAGCCTTTCTGT
  GAACCAGATGTGGTTTGGGGCAGATCTGTAGTAAACAATGGTGATTTTATTTATTTTTACTCTCTGGAAA
  AGGAGATAATACAATTCCAGAAAGTGAACTCATATTTCTAAGGTTAAGATTCCCTTTTATTGCACCTAGA
  ATAGTGCTATGCACAGAGCGGGTGCTTGAGTTGTTGTCGTTTTTTGTTTGTTTTTTAAATGTAAACTGGT
  AAATTTTGTGCTTATCTTCAAGGCTGGCTTAAGTATAAAATTGTTTTTTAAACACTTGAAAAATTAAAGG
  ATTTGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001005861.2
• Summary: The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]
• Locus ID: 6259