MBD1 (NM_002384) Human 3' UTR Clone

CAT#: SC211938

3`UTR clone of methyl-CpG binding domain protein 1 (MBD1) transcript variant 4 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MBD1
• Synonyms: CXXC3; PCM1; RFT
• ACCN: NM_002384
• Insert Size: 1001 bp
• Sequence Data:
  Insert Sequence

  >SC211938 3'UTR clone of NM_002384
  The sequence shown below is from the reference sequence of NM_002384. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACCTGGAGCTAGAAAGCAGTAGACTGGAGGCTTCTACAGACTGTAGGATTCAAGTCTGCAGGGCAGGCAC
  TCGGGAAGGGAAGATGGATGTAAAGTGTGGGAGACCGAGGACACAGTGGAGCCCACGAGCACGAGCTGGA
  ACCCACGAGGATGGCCTGGAACCCATGTCAGTCTCTCACCACCTCCAGCTTCGATGATGTGGGTGTCCTG
  CAGAAGAAGCTGGTGCCCTTCCTCACAGAGTTAAATATGCATCTGGCCCAGGAATTAGAGAAGCTGAAAG
  GATGATCCTGGGGAAGGTGGAGCAGCTGCAGGCCTGGCTGCAGGCCTGACTACTGCCCACACCAACGAGG
  TGATCTAGCAGATACATGGCAACGTGTGAACTGCAACAACGCCTGGTGCCCCAGCACCAACCTTCCAAGT
  GTAAAAACAATGTGCTGCTGCTTCACTTCCGCCCTCCGGTTATCAAGCAAAATGTCTCTTGTGGCCCATC
  TTACTGGAAGAGAGTTCCGGGAAACATAGCCTCACCAAGGTGACACATTACAAAGCCACCCTACCATGAA
  TCCGCTCCCAAGGGTCTCACTGCTCACCTGAGGATAACTCAATATAACTATGTGGCTGAAAATGCAAAGC
  TGAAGACCATGGATTTCATGGTGATTCCAGCAAGTACAGAGATTCTATGAAGCCCACCCAGAAAAAACTT
  GCTGGTCCTGGCTATTTTTGTGTCATTTATTCAAGTATTGAGAACCTGGCCTGTGGTAGGCACTGTACTT
  AATACTAGGATACAGAAATGCAAAAGATACGGCCCATGCAATTTTATTAAATGCATCAATATGTATTACA
  AATGGTGAATGGATTTCCAACTTTATCATGGAATTTAATGCTGAATATATAGAATTCAGAAAATTGTTGG
  GAGGACAGCCCTTTTGTGAACCTTGTTTGGGGCACAGTAGGAATTGGAAATAATTTAGTTTCTATCTCTA
  AGCTGTTCTATTTTAAAATTA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002384.2
• Summary: 'The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011]'
• Locus ID: 4152