MBD1 (NM_015847) Human 3' UTR Clone
CAT#: SC211939
3`UTR clone of methyl-CpG binding domain protein 1 (MBD1) transcript variant PCM1 for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | MBD1 |
Synonyms | CXXC3; PCM1; RFT |
ACCN | NM_015847 |
Insert Size | 1001 bp |
Sequence Data |
>SC211939 3'UTR clone of NM_015847
The sequence shown below is from the reference sequence of NM_015847. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon CAATTGGCAGAGCTCAGAATTCAAGCGATCGC ACCTGGAGCTAGAAAGCAGTAGACTGGAGGCTTCTACAGACTGTAGGATTCAAGTCTGCAGGGCAGGCAC TCGGGAAGGGAAGATGGATGTAAAGTGTGGGAGACCGAGGACACAGTGGAGCCCACGAGCACGAGCTGGA ACCCACGAGGATGGCCTGGAACCCATGTCAGTCTCTCACCACCTCCAGCTTCGATGATGTGGGTGTCCTG CAGAAGAAGCTGGTGCCCTTCCTCACAGAGTTAAATATGCATCTGGCCCAGGAATTAGAGAAGCTGAAAG GATGATCCTGGGGAAGGTGGAGCAGCTGCAGGCCTGGCTGCAGGCCTGACTACTGCCCACACCAACGAGG TGATCTAGCAGATACATGGCAACGTGTGAACTGCAACAACGCCTGGTGCCCCAGCACCAACCTTCCAAGT GTAAAAACAATGTGCTGCTGCTTCACTTCCGCCCTCCGGTTATCAAGCAAAATGTCTCTTGTGGCCCATC TTACTGGAAGAGAGTTCCGGGAAACATAGCCTCACCAAGGTGACACATTACAAAGCCACCCTACCATGAA TCCGCTCCCAAGGGTCTCACTGCTCACCTGAGGATAACTCAATATAACTATGTGGCTGAAAATGCAAAGC TGAAGACCATGGATTTCATGGTGATTCCAGCAAGTACAGAGATTCTATGAAGCCCACCCAGAAAAAACTT GCTGGTCCTGGCTATTTTTGTGTCATTTATTCAAGTATTGAGAACCTGGCCTGTGGTAGGCACTGTACTT AATACTAGGATACAGAAATGCAAAAGATACGGCCCATGCAATTTTATTAAATGCATCAATATGTATTACA AATGGTGAATGGATTTCCAACTTTATCATGGAATTTAATGCTGAATATATAGAATTCAGAAAATTGTTGG GAGGACAGCCCTTTTGTGAACCTTGTTTGGGGCACAGTAGGAATTGGAAATAATTTAGTTTCTATCTCTA AGCTGTTCTATTTTAAAATTA ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Reference Data | |
RefSeq | NM_015847.3 |
Summary | 'The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011]' |
Locus ID | 4152 |
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