HERC4 (NM_015601) Human 3' UTR Clone

CAT#: SC212050

3`UTR clone of hect domain and RLD 4 (HERC4) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HERC4
• Synonyms: DKFZp564G092; KIAA1593
• ACCN: NM_015601
• Insert Size: 1043
• Sequence Data:
  Insert Sequence

  >SC212050 3'UTR clone of NM_015601
  The sequence shown below is from the reference sequence of NM_015601. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GATCACAATGAAGGCTTCAGTTTAATATAACTTTGGAGTTATAACTATTCAGTTTAGTGCAAAAGCATTA
  AACTATTTGTGTTTTTCTTGTGGTGATGAATTCAGCAAGGTGACAGAGGTACTATTATAATTCTTACTTG
  CAGAATGTTCAATCTACGAGTGTTCATGGAAGCCAAAAAATATTAAAGGAAAATGAACAAACTGTTAATA
  TTATTGTACAGAACCATGGATTTTTTTTGACCATCTTCTAATAAACATAGCAAGTATTATGAATACATTA
  AAGTTTTACTAACATGAATTTTAAGAGTTTGCATATTTCAGAAATGATCTGGTGTGAGTGCATGGAAATA
  TTGCTTAATTTTTCTTCAATCATTGAGTGAAAAACCTTTAACTTTGGCCTGCAATAGTCATTTGATTATT
  TTTTCATTTTGTAAATAATGTTAAGTTTTGTAATAAAATAGTTATGTTCTGATACCAGTACAGTTTCTAT
  GGTTGTAATTGAACTTGAACAGACTTTTAAAGGTTAAAAATTATGATTTAAATCTTACTCTGAGACTCTA
  TAAAAAGAAAAAAAAGGTAGCATGGTGGAAACACTATCTTTTCTTTTTTGCTAGAATAAGTGTCTTTGTG
  CAAACCTAAATCACAGATAGGGAATATACTACATAACCTGCAGTTCTTTTCTTTGTGAATCCTGACACAC
  TGATAGATGGGGGATTGTCGATCAGAGAACTTATTAATATTTAGTACTGGAAGAACTCTGTCTCCACAGT
  TGCCAGTAATAAAAAGAAACATTGGCTACTATGAGCACCAATCACTGGGTTATAGCTTTCAAAATTATTG
  ATGCTGCAGTGCTTTAGAGCTATTTCCTTGAATTTAAGAAACAAATCTTAACAGTTTTATGGTGCTGATG
  CTTAGTTGTCTCATGCCATTAAATTGTAAAAGTGAGTTGATGCAATACATGTGACTTTCTGCTATAATGC
  AAATATTTATTTTTTAAATTTATTTTAAAATGCCGTGAAAACTGTTTAATAAAGATTTATTGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_015601.3
• Summary: HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]). [supplied by OMIM, Mar 2008]
• Locus ID: 26091