CD247 (NM_000734) Human 3' UTR Clone

CAT#: SC212071

3`UTR clone of CD247 molecule (CD247) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CD247
• Synonyms: CD3-ZETA; CD3H; CD3Q; CD3Z; IMD25; T3Z; TCRZ
• ACCN: NM_000734
• Insert Size: 1060 bp
• Sequence Data:
  Insert Sequence

  >SC212071 3'UTR clone of NM_000734
  The sequence shown below is from the reference sequence of NM_000734. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GACACCTACGACGCCCTTCACATGCAGGCCCTGCCCCCTCGCTAACAGCCAGGGGATTTCACCACTCAAA
  GGCCAGACCTGCAGACGCCCAGATTATGAGACACAGGATGAAGCATTTACAACCCGGTTCACTCTTCTCA
  GCCACTGAAGTATTCCCCTTTATGTACAGGATGCTTTGGTTATATTTAGCTCCAAACCTTCACACACAGA
  CTGTTGTCCCTGCACTCTTTAAGGGAGTGTACTCCCAGGGCTTACGGCCCTGGCCTTGGGCCCTCTGGTT
  TGCCGGTGGTGCAGGTAGACCTGTCTCCTGGCGGTTCCTCGTTCTCCCTGGGAGGCGGGCGCACTGCCTC
  TCACAGCTGAGTTGTTGAGTCTGTTTTGTAAAGTCCCCAGAGAAAGCGCAGATGCTAGCACATGCCCTAA
  TGTCTGTATCACTCTGTGTCTGAGTGGCTTCACTCCTGCTGTAAATTTGGCTTCTGTTGTCACCTTCACC
  TCCTTTCAAGGTAACTGTACTGGGCCATGTTGTGCCTCCCTGGTGAGAGGGCCGGGCAGAGGGGCAGATG
  GAAAGGAGCCTAGGCCAGGTGCAACCAGGGAGCTGCAGGGGCATGGGAAGGTGGGCGGGCAGGGGAGGGT
  CAGCCAGGGCCTGCGAGGGCAGCGGGAGCCTCCCTGCCTCAGGCCTCTGTGCCGCACCATTGAACTGTAC
  CATGTGCTACAGGGGCCAGAAGATGAACAGACTGACCTTGATGAGCTGTGCACAAAGTGGCATAAAAAAC
  ATGTGGTTACACAGTGTGAATAAAGTGCTGCGGAGCAAGAGGAGGCCGTTGATTCACTTCACGCTTTCAG
  CGAATGACAAAATCATCTTTGTGAAGGCCTCGCAGGAAGACCCAACACATGGGACCTATAACTGCCCAGC
  GGACAGTGGCAGGACAGGAAAAACCCGTCAATGTACTAGGATACTGCTGCGTCATTACAGGGCACAGGCC
  ATGGATGGAAAACGCTCTCTGCTCTGCTTTTTTTCTACTGTTTTAATTTATACTGGCATGCTAAAGCCTT
  CCTATTTTGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000734.3
• Summary: 'The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 919