TLR5 (NM_003268) Human 3' UTR Clone

CAT#: SC212140

3`UTR clone of toll-like receptor 5 (TLR5) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TLR5
• Synonyms: MELIOS; SLE1; SLEB1; TIL3
• ACCN: NM_003268
• Insert Size: 1070 bp
• Sequence Data:
  Insert Sequence

  >SC212140 3'UTR clone of NM_003268
  The sequence shown below is from the reference sequence of NM_003268. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAAACTGTAGCAACCATCTCCTAATCAAAGGAGCAATTTCCAACTTATCTCAAGCCACAAATAACTCTTC
  ACTTTGTATTTGCACCAAGTTATCATTTTGGGGTCCTCTCTGGAGGTTTTTTTTTTCTTTTTGCTACTAT
  GAAAACAACATAAATCTCTCAATTTTCGTATCAACACCATGTTCTGTCTCACTAACCTCCAAATGGAAAA
  TAATAGATCTAGAAAATTGCAACTGCCCTTAGAGGTTTCCAGTCTCCATTGATTTTCTTTCAGATCCAAT
  AATACCGTTCTGTCCTGCTGTGTTGATTATGGAATGTATCCTAATCATGGGAAGGGCACCTTGGGAGAAG
  TTGCAGATGGCTGACGTGCTTTCTCTAGCATTCAGCTAAAAAATGGGATGGTCCATGATTCTGCGTTCTC
  TGTGTTCTGCAAAACAACATTAAGTAGAAAACAAACAGAAGCAGAGGCACATTTCCTTCTTTTGCCACAG
  AAACAATGCCACTGTTGAGTGCAAGTCACACTTTGTCTCATAGATAAGAGGTGGCCCCAAAGAAGCTGGG
  ATGAATGAGTCACTACCTGTCTTGTGACTGTACTGCACTTCATGTTCTTACCTTCGGCTTCTCCAGGTCT
  CGCCCTAGTGAGCCAAGAACTTTCTCTCACATGCTGCCTTTATTTAAGCCTGGCCTTCAAGACCTTCCAT
  GATTTATCACCAACCTACCTTTTCAGCTTTGTTTCCTAGCACACCTCATTGATCAGCTGCTCAGGCTGTT
  GCATGAAGAACATGGACTTTGCACACAGATCCTGGGTTGAGTTCTGAGTCAGCTGTGTATTAGCCATGTA
  ACCTTGGCCTTGGTATGCCCTTGCTGGACCTACATTTTCTCACGTAACAGCATCTATGTCATAGAATTCC
  TGTGAAAATTAAATTGGCCAAGGATGTCAGGGCTTCTCAGATCTTTTCCTTCGTTGCCCTAATGACCACA
  AGAGAACACATACACTGAAGGCCTCCTGGGGGGCAGTTGCAATTTCACTGAAGTTGTATTTTTTTATCTT
  AAATGAAATCTATGTGTATT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003268.5
• Summary: 'This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.[provided by RefSeq, Dec 2009]'
• Locus ID: 7100