Oncostatin M (OSM) (NM_020530) Human 3' UTR Clone

CAT#: SC212211

3`UTR clone of oncostatin M (OSM) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: OSM
• Synonyms: MGC20461
• ACCN: NM_020530
• Insert Size: 1074 bp
• Sequence Data:
  Insert Sequence

  >SC212211 3'UTR clone of NM_020530
  The sequence shown below is from the reference sequence of NM_020530. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGAAAGGCAAGAGACTCATGACCAGGGGACAGCTGCCCCGGTAGCCTCGAGAGCACCCCTTGCCGGTGAA
  GGATGCGGCAGGTGCTCTGTGGATGAGAGGAACCATCGCAGGATGACAGCTCCCGGGTCCCCAAACCTGT
  TCCCCTCTGCTACTAGCCACTGAGAAGTGCACTTTAAGAGGTGGGAGCTGGGCAGACCCCTCTACCTCCT
  CCAGGCTGGGAGACAGAGTCAGGCTGTTGCGCTCCCACCTCAGCCCCAAGTTCCCCAGGCCCAGTGGGGT
  GGCCGGGCGGGCCACGCGGGACCGACTTTCCATTGATTCAGGGGTCTGATGACACAGGCTGACTCATGGC
  CGGGCTGACTGCCCCCCTGCCTTGCTCCCCGAGGCCTGCCGGTCCTTCCCTCTCATGACTTGCAGGGCCG
  TTGCCCCCAGACTTCCTCCTTTCCGTGTTTCTGAAGGGGAGGTCACAGCCTGAGCTGGCCTCCTATGCCT
  CATCATGTCCCAAACCAGACACCTGGATGTCTGGGTGACCTCACTTTAGGCAGCTGTAACAGCGGCAGGG
  TGTCCCAGGAGCCCTGATCCGGGGGTCCAGGGAATGGAGCTCAGGTCCCAGGCCAGCCCCGAAGTCGCCA
  CGTGGCCTGGGGCAGGTCACTTTACCTCTGTGGACCTGTTTTCTCTTTGTGAAGCTAGGGAGTTAGAGGC
  TGTACAAGGCCCCCACTGCCTGTCGGTTGCTTGGATTCCCTGACGTAAGGTGGATATTAAAAATCTGTAA
  ATCAGGACAGGTGGTGCAAATGGCGCTGGGAGGTGTACACGGAGGTCTCTGTAAAAGCAGACCCACCTCC
  CAGCGCCGGGAAGCCCGTCTTGGGTCCTCGCTGCTGGCTGCTCCCCCTGGTGGTGGATCCTGGAATTTTC
  TCACGCAGGAGCCATTGCTCTCCTAGAGGGGGTCTCAGAAACTGCGAGGCCAGTTCCTTGGAGGGACATG
  ACTAATTTATCGATTTTTATCAATTTTTATCAGTTTTATATTTATAAGCCTTATTTATGATGTATATTTA
  ATGTTAATATTGTGCAAACTTATA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_020530.3
• Summary: 'This gene encodes a member of the leukemia inhibitory factor/oncostatin-M (LIF/OSM) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein is a secreted cytokine and growth regulator that inhibits the proliferation of a number of tumor cell lines. This protein also regulates the production of other cytokines, including interleukin 6, granulocyte-colony stimulating factor and granulocyte-macrophage colony stimulating factor in endothelial cells. This gene and the related gene, leukemia inhibitory factor, also present on chromosome 22, may have resulted from the duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]'
• Locus ID: 5008