CNOT2 (NM_014515) Human 3' UTR Clone

CAT#: SC212273

3`UTR clone of CCR4-NOT transcription complex subunit 2 (CNOT2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CNOT2
• Synonyms: CDC36; HSPC131; NOT2; NOT2H
• ACCN: NM_014515
• Insert Size: 1078 bp
• Sequence Data:
  Insert Sequence

  >SC212273 3'UTR clone of NM_014515
  The sequence shown below is from the reference sequence of NM_014515. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACTACAACCCTGCTCAGCAAGCCTTCTAAAAAAAAAAAAAAAAAAAAAAAAAGACTTCCCTTTTCTTGGG
  GTATGGCTGTCTCAGCACAATACTCAACATAACTGCAGAACTGATGTGGCTCAGGCACCCTGGTTTTAAT
  TCCTTGAGGATCTGGCAATTGGCTTACGCAAAAGGTCACCATTTGAGGTCCTGCCTTACTAATTATGTGC
  TGCCCAACAACTAAATTTGTAATTTGTTTTTCTCTAGTTTGAGCAGGGTCTGAATTTTTTCATTTATTTC
  CTTTTTTGCCAGCAGACAGACTTGAGTCTGTAAAGACAAGCAAATACACTGACAGAAGTTTACCATAGTT
  TCTAAAATGTAAAAAAGAAAACCCCCAAAAGACTCAAGAAAATTAGACCACAAATTTTGCATTGTTCATT
  GTAGCACTATTGGTAATAAAATAACAAATGTTTGTGCATTTTTATGTGAAGATCCTTCTCGTATTTCATT
  TGGAAAGATGAGCAAGAGGTCTGCTTCCTTCATTTTACTTCCCCTTCTGTTTTTGAAAGGCAGTTTCGCC
  AAGCTTAATGCAAGAATATCTGACTGTTTAGAAGAAAGATATTGCCACAATCTCTGGATGGTTTTCCAGG
  GTTGTGTTATTACTGAGCTTCATCTTTCCAGAATGAGCAAAACACTGTCCAGTCTTTGTTACGATTTTGT
  AATAAATGTGTACATTTTTTTTAAATTTTTGGACATCACATGAATAAAGGTATGTATGTACGAATGTGTA
  TATATTATATATATGACATCTATTTTGGAAAATGTTTGCCCTGCTGTACCTCATTTTTAGGAGGTGTGCA
  TGGATGCAATATATGAAAATGGGACATTCTGGAACTGCTGGTCAGGGGACTTTGTCGCCCTGTGCACTAA
  AAGGGCCAGATTTTCAGCAGCCAAGGACATCCATACCCAAGTGAATGTGATGGGACTTAAAAGAAGTGAA
  CTGAGACAATTCACTCTGGCTGTTTGAACAGCAGCGTTTCATAGGAAGAGAAAAAAAGATCAATCTTGTA
  TTTTCTGACCACATAAAGGCTTCTTCTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_014515.4
• Summary: 'This gene encodes a subunit of the multi-component CCR4-NOT complex. The CCR4-NOT complex regulates mRNA synthesis and degradation and is also thought to be involved in mRNA splicing, transport and localization. The encoded protein interacts with histone deacetylases and functions as a repressor of polymerase II transcription. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010]'
• Locus ID: 4848