RAP1A (NM_002884) Human 3' UTR Clone

CAT#: SC212276

3`UTR clone of RAP1A member of RAS oncogene family (RAP1A) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RAP1A
• Synonyms: C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21
• ACCN: NM_002884
• Insert Size: 1095 bp
• Sequence Data:
  Insert Sequence

  >SC212276 3'UTR clone of NM_002884
  The sequence shown below is from the reference sequence of NM_002884. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCATGTCTGCTGCTCTAGGCCCATAGTCAGCAGCAGCTCTGAGCCAGATTACAGGAATGAAGAACTGTTG
  CCTAATTGGAAAGTGCCAGCATTCCAGACTTCAAAAATAAAAAATCTGAAGAGGCTTCTCCTGTTTTATA
  TATTATGTGAAGAATTTAGATCTTATATTGGTTTGCACAAGTTCCCTGGAGAAAAAAATTGCTCTGTGTA
  TATCTCTTGGAAAATAAGACAATAGTATTTCTCCTTTGCAATAGCAGTTATAACAGATGTGAAAATATAC
  TTGACTCTAATATGATTATACAAAAGAGCATGGATGCATTTCAAATGTTAGATATTGCTACTATAATCAA
  ATGATTTCATATTGATCTTTTTATCATGATCCTCCCTATCAAGCACTAAAAAGTTGAACCATTATACTTT
  ATATCTGTAATGATACTGATTATGAAATGTCCCCTCAAACTCATTGCAGCAGATAACTTTTTTGAGTCAT
  TGACTTCATTTTATATTTAAAAAATTATGGAATATCATCTGTCATTATATTCTAATTAAAATTGTGCATA
  ATGCTTTGGAAAAATGGGTCTTTTATAGGAAAAAAACTGGGATAACTGATTTCTATGGCTTTCAAAGCTA
  AAATATATAATATACTAAACCAACTCTAATATTGCTTCTTGTGTTTTACTGTCAGATTAAATTACAGCTT
  TTATGGATGATTAAATTTTAGTACATTTTCATTTGGTTTGTGTGTTTTTGTTATTGTTTATAGATTAAAG
  CGTTTATTTTATAATGACCACATTGTTTTAAATGCGACAGTAGCTCCTTTCTGCCTAGTATCTGCAGAAC
  ACTGGCTTTAAACTATACTAAGTAACTGGTGATTTCTCTAGGAACAGACCTCGCACTTTCTGTTCTAAAT
  ATATTTATTCCTACTATACAGTAAAATACATCAGACAACATAGAATAGTTTTGTATATTCTCTCTTGATC
  TTAAAGATTGTATCTTATTGAATATCCCACTGTATATTATTTTTATATGTAAAAAGATAAGTTTAATACT
  GTATCAGGGTTTAACTTTTACTATTTCAGATCTTCCACAGCTTGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002884.2
• Summary: 'This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]'
• Locus ID: 5906