CRF1 (CRHR1) (NM_001145148) Human 3' UTR Clone

CAT#: SC212297

3`UTR clone of corticotropin releasing hormone receptor 1 (CRHR1) transcript variant 4 for miRNA target validation

Reconstitution Protocol

USD 560.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol CRHR1
Synonyms CRF-R; CRF-R-1; CRF-R1; CRF1; CRFR-1; CRFR1; CRH-R-1; CRH-R1; CRHR; CRHR1L
ACCN NM_001145148
Insert Size 1069 bp
Sequence Data
>SC212297 3'UTR clone of NM_001145148
The sequence shown below is from the reference sequence of NM_001145148. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

TCACAGCATCAAGCAGTCCACAGCAGTCTGAGCTGGCAGGTCATGGAGCAGCCCCCAAAGAGCTGTGGCT
GGGGGGATGACGGCCAGGCTCCCTGACCACCCTGCCTGTGGAGGTGACCTGTTAGGTCTCATGCCCACTC
CCCCAGGAGCAGCTGGCACTGACAGCCTGGGGGGGCCGCTCTCCCCCTGCAGCCGTGCAGGACTCTAGCT
CATGAGTGGAAAGTCACCTACAGGACTGGGCCGGGCCCAGGGCCTCTGGCTTCCCTGCCCAATCCTCCCT
GGAGAAGGGACATGGGAATGAATTGAAATGGGGCGCTGGACACCTACAGCAGCACGCATGTCCCTCCAAG
GCTGTCTTCTCCCAGAGCACAAGAAGGCCAGCCCACTGGGCCCTGGGGCTGCCCTCGGCAACCGTGGGGA
GGCCATTTGCTGCCCTGGGGCATCATGGGCAACTCGTGACAGCCTCTGACTCACCACGATGACGCCTCTG
GACCTCGGTGATGCCTTCCGACACCACTGGGAACCAAGGGCCCTCACTCAGGAACCCTGGAGACAGAAGT
CAGGTGTCATCATCAGACTTGCGGCCACAGCACTAGAGTCACCCCCCCAGGCCTCCAGAACCTTACTGGC
ACTGTGGCACTGCCACCAGCAATGCCCTGCCTTGCTGCCTTCACCCTGAACATTTAGTACCCTGCAGGCC
AGGCCAGCTTCCCCTCACTTAACCACCCCATACCAGTCACCTCCTGCTCCTTTTCCTCTTTTGTGAGAAG
ATGGGGGCTGGAGGGGGCAGAGTGGCCTGTGAGCAAGAGCCAGGGGTGTCCCAGTCCCAGCCTCTGGGGC
AGAGCTTGTAGCCCTGGATGGCCTCTGGGGCAGGACCACTAGCTAAGCAAGCCAGGAGAAGACCCCTGCC
CAAGTGGCTCTTGGGACAACGTGCTGCTTACACTCCAGGTGTGGACCGGCCGCAGCCCCCACTGACCTGC
CCATGTCCAGAGGGACTGGACAGCCAGGGCAGGGCTTTGGGGGGCACTAGAAGATGAGGGTGTCGGCTGT
GAGGCGGGTGGCTGGTATA

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Reference Data
RefSeq NM_001145148.1
Summary 'This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants. Naturally-occurring readthrough transcription between this gene and upstream GeneID:147081 results in transcripts that encode isoforms that share similarity with the products of this gene. [provided by RefSeq, Aug 2016]'
Locus ID 1394

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.