Tryptophanyl tRNA synthetase (WARS) (NM_173701) Human 3' UTR Clone

CAT#: SC212343

3`UTR clone of tryptophanyl-tRNA synthetase (WARS) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: WARS
• Synonyms: GAMMA-2; HMN9; IFI53; IFP53
• ACCN: NM_173701
• Insert Size: 1086 bp
• Sequence Data:
  Insert Sequence

  >SC212343 3'UTR clone of NM_173701
  The sequence shown below is from the reference sequence of NM_173701. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGCTGTCCTTCGACTTTCAGTAGCACTCGTTTTACATATGCTTATAAAAGAAGTGATGTATCAGTAATGT
  ATCAATAATCCCAGCCCAGTCAAAGCACCGCCACCTGTAGGCTTCTGTCTCATGGTAATTACTGGGCCTG
  GCCTCTGTAAGCCTGTGTATGTTATCAATACTGTTTCTTCCTGTGAGTTCCATTATTTCTATCTCTTATG
  GGCAAAGCATTGTGGGTAATTGGTGCTGGCTAACATTGCATGGTCGGATAGAGAAGTCCAGCTGTGAGTC
  TCTCCCCAAAGCAGCCCCACAGTGGAGCCTTTGGCTGGAAGTCCATGGGCCACCCTGTTCTTGTCCATGG
  AGGACTCCGAGGGTTCCAAGTATACTCTTAAGACCCACTCTGTTTAAAAATATATATTCTATGTATGCGT
  ATATGGAATTGAAATGTCATTATTGTAACCTAGAAAGTGCTTTGAAATATTGATGTGGGGAGGTTTATTG
  AGCACAAGATGTATTTCAGCCCATGCCCCCTCCCAAAAAGAAATTGATAAGTAAAAGCTTCGTTATACAT
  TTGACTAAGAAATCACCCAGCTTTAAAGCTGCTTTTAACAATGAAGATTGAACAGAGTTCAGCAATTTTG
  ATTAAATTAAGACTTGGGGGTGAAACTTTCCAGTTTACTGAACTCCAGACCATGCATGTAGTCCACTCCA
  GAAATCATGCTCGCTTCCCTTGGCACACCAGTGTTCTCCTGCCAAATGACCCTAGACCCTCTGTCCTGCA
  GAGTCAGGGTGGCTTTTCCCCTGACTGTGTCCGATGCCAAGGAGTCCTGGCCTCCGCAGATGCTTCATTT
  TGACCCTTGGCTGCAGTGGAAGTCAGCACAGAGCAGTGCCCTGGCTGTGTCCCTGGACGGGTGGACTTAG
  CTAGGGAGAAAGTCGAGGCAGCAGCCCTCGAGGCCCTCACAGATGTCTAGGCAGGCCTCATTTCATCACG
  CAGCATGTGCAGGCCTGGAAGAGCAAAGCCAAATCTCAGGGAAGTCCTTGGTTGATGTATCTGGGTCTCC
  TCTGGAGCACTCTGCCCTCCTGTCACCCAGTAGAGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_173701.1
• Summary: 'Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 7453