DNA Ligase IV (LIG4) (NM_001098268) Human 3' UTR Clone

CAT#: SC212468

3`UTR clone of ligase IV DNA ATP-dependent (LIG4) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: LIG4
• Synonyms: LIG4S
• ACCN: NM_001098268
• Insert Size: 1109 bp
• Sequence Data:
  Insert Sequence

  >SC212468 3'UTR clone of NM_001098268
  The sequence shown below is from the reference sequence of NM_001098268. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAATAGACAAGTGTGAATTACAAGAAGAAAACCAGTATTTGATTTAAAGCTAGGTTTCCTAGTGAGGAAA
  GCCTCTGATCTGGCAGACTCATTGCAGCAGGTGGTAATGATAAAATACTAAACTACATTTTATTTTTGTA
  TCTTAAAAATCTATGCCTAAAAAGTATCATTACATATAGGAAAACAATAATTTTAACTTTTAAGGTTGAA
  AAGACAATAGCCCAAAGCCAAGAAAGAAAAATTATCTTGAATGTAGTATTCAATGATTTTTTATGATCAA
  GGTGAAATAAACAGTCTAAAGAAGAGGTGTTTTTATAATATCCATATAGAAATCTAGAATTTTTACTTAG
  ATACTAATAAAATACATTTAGAAACTTTTAAAGTCATGAAAAAGCATTAACCTTCTAAACAGTATATTCT
  AAAAAGTCAAAACGTTAACAATAGTTTTTATCTAATAAAAGCACTGCAAGAAAATAGGGTAGAATTGTTA
  CAGCTGGACTTGTAAAAATATGTCTTTTTACTCAGGGTTTAAAATGTCCCATTTAAATATGAAATGTAAA
  CAAATTTGTTTTTTAAGGTTAAGGCCAAATGTAACAATAAAACCCTGTCGATGGTTTTAGCTAAATTAGA
  GGAAGTTGTATGAGACTTAATGATCTAAAAACTTAAAATTGAATTGGTTTGATTAAAAATAAAGCTTGCA
  ATTTTAAAAGTAGCTCACATTTAATTTCTTGTGTGAAATAGAACATGCTTTAAAGGAAGTATTTTTATGT
  GAATTTGCATTCCAGTATAAATAGTATTCACAAAAAAGATTTTCCTAGATTTTATCTATTGAATAGGTGT
  CAATATGGCATGCATATTGTAACTTTCATTAGAAATAAGTTGCTTTGACTTTTAAAAATGACATAGTTAG
  ATTATTTAAAGTCAATGTATATAGTATATATTATGTATGGATTTATATACCAAATTTTGGAATACAGCCT
  ATCTCATGACCATATTGAAATGTACGGAATTTGATCCATGCGATACTATGTGTGCATTATTTGAAAGTTA
  TTGGAAATTTTATTCAAACCGTGGAACAAATGTATGTGATTTTGTTATACTTCTTAATT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001098268.1
• Summary: 'The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]'
• Locus ID: 3981