CNOT4 (NM_013316) Human 3' UTR Clone

CAT#: SC212471

3`UTR clone of CCR4-NOT transcription complex subunit 4 (CNOT4) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CNOT4
• Synonyms: CLONE243; NOT4; NOT4H
• ACCN: NM_013316
• Insert Size: 1107 bp
• Sequence Data:
  Insert Sequence

  >SC212471 3'UTR clone of NM_013316
  The sequence shown below is from the reference sequence of NM_013316. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCAACACTGGACCGCCATTAGGCAAAGAGGAGCAACCATTAGAAAATGCAGGATTTGTCCCACTCTGTTT
  TCTCCCTCTCAGCCCACCACCCACAGCTCCCTTCTCATCTCTTATGTTCTGAAGAATCCAGTACCTGATC
  AATTTTTTTTCTCCCTAACCCCAGATGCAATGCGATCACAGGGTCATTACCATCTCTTTATTAATTGTAA
  AAATTTTTGTTGATCCAGCATTTGAGTGACACTGTTGAATGTTTCTAAAAATGCCTTTTTAAGGAGAGAA
  AAAAAAATCAAAGGGCAGTCTCAATACTTAGAAAATTATTGTTTGTCTGTTGCGCATAATAATGACATAA
  TCTGCTTAGCAGAAAATGACGATTAATCTATAGGAAAGCTCAAGTAAATGCATTATCAACTGCAGAAGTT
  TGAAAACCAGGTTCATTTACGTGAGATTGCTAAATGCATGGGGGAAAGCAGTGGTCCTAGCATCCATCTT
  GTATTCAGCTTATCATTATTGCAGGGAAAATGCTTTTAATTTAAATTAATTTTTAATTCTTTTGGCAAGT
  TGATGGCAAGGACTTGATTGTGTCATTAAGCAAAAGAATGTATTGGAAGTTGATGGAAAGACAAATTCAT
  CTGTAGTAGTAACTGGCCGATTGCTAAAGAGTTCATAAGGAGGTGAGAAGTAATTTTTTTAAAGGAGAAA
  AATTTTTTGGCTTTAGATTTAAAGTAAATTGAAATGTTTTAAAGAAAAAAGTATTCACAGATTTAATACC
  TATTAATAATATAAGAGCTGAAATGTAAGTCATTTCTTCAGTCCTTCTCCTCTGTCGGAATCTTTTTTGT
  TTTACCATAAATTCACCTGACGAGGGCACTCTGAGATAGCACTGCTCTGGGGCCATCTGATCACCATCGG
  GAGCAAATCTCTGACCTCCTTGCCTGCAGCTTTTACTTAACCCTGTAGTTTCTGGACGTTTGTGCAGTAT
  TGAAAAGACAGGAGAAAAGCAAACAGAAACCTGGTTATAACCTGACGCTAAAACTAAAAACAAGGAAATG
  TACCTCTTTCTTCAGAATTAAAACTAAAATCTTAAATAAAACAGAAAACTTGATGAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_013316.2
• Summary: 'The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]'
• Locus ID: 4850